Soni Pankaj, Singh Manpreet, Kamble Ashwini L, Banerjee Uttam C
Biocatalysis Laboratory, Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector 67, S.A.S. Nagar 160 062, Punjab, India.
Bioresour Technol. 2007 Mar;98(4):829-33. doi: 10.1016/j.biortech.2006.03.008. Epub 2006 May 11.
Culture conditions were optimized for the growth and carbonyl reductase production by a novel yeast strain Candida viswanathii. Response surface methodology was applied for the critical medium components (initial pH, mannitol, yeast extract and calcium chloride) identified earlier by one-factor-at-a-time approach. Central composite design was used for the optimization studies. Using this methodology, the optimal values for the concentration of mannitol, initial pH, yeast extract and calcium chloride were 1.9, 7.5, 1.6 and 4, respectively. This medium was projected to produce, theoretically, growth having an optical density of 1.1 (600 nm) and an enzyme activity of 81.5 U/ml. Using this optimized medium, an experimental growth of 1.1 OD (600 nm) and enzyme activity 80.9 U/ml verified the applied methodology. This approach for medium optimization led to an enhancement of the growth and enzyme activity by 1.3 and 2.3 times higher, respectively, as compared to the unoptimized media.
针对一种新型酵母菌株维斯瓦纳特念珠菌（Candida viswanathii）的生长和羰基还原酶生产，对培养条件进行了优化。响应面法应用于通过一次单因素法先前确定的关键培养基成分（初始pH值、甘露醇、酵母提取物和氯化钙）。采用中心复合设计进行优化研究。使用该方法，甘露醇浓度、初始pH值、酵母提取物和氯化钙的最佳值分别为1.9、7.5、1.6和4。理论上，该培养基预计可产生光密度为1.1（600nm）的生长和81.5U/ml的酶活性。使用这种优化培养基，1.1OD（600nm）的实验生长和80.9U/ml的酶活性验证了所应用的方法。与未优化的培养基相比，这种培养基优化方法分别使生长和酶活性提高了1.3倍和2.3倍。
