Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, 221 00, Lund, Sweden.
Mar Biotechnol (NY). 2010 Oct;12(5):586-93. doi: 10.1007/s10126-009-9246-6. Epub 2009 Dec 3.
Two cultivation steps were used for production of biomass and ectoine by Halomonas boliviensis, respectively. The optimization of some nutrient parameters in each step was investigated by using response surface methodology. Twenty and 12 experiments were performed to attain optimal conditions for biomass and ectoine production, respectively. The model predicted a maximum biomass concentration of 3.34 g/L on optimization of NH(4)Cl, K(2)HPO(4), and MgSO(4)•7H(2)O concentrations during the first cultivation, while a maximum ectoine concentration of 1.27 g/L was predicted on optimizing NaCl and monosodium glutamate concentrations in the second cultivation. The experimental values obtained (3.36 g biomass/L and 1.25 g ectoine/L) were in good agreement with the predicted values. The optimized conditions were also used for two-step 1.5-L fed-batch fermentations. In the first step, biomass concentration of 28.7 g/L was obtained while in the second step biomass concentration increased to 63 g/L. Ectoine concentration of 9.2 g/L was obtained, and the overall ectoine productivity was 6.3 g/L/day, being among the highest reported so far.
分别采用两步培养法来生产盐单胞菌（Halomonas boliviensis）的生物量和四氢嘧啶。利用响应面法对每一步中的一些营养参数进行了优化。分别进行了 20 次和 12 次实验，以获得生物量和四氢嘧啶生产的最佳条件。模型预测在优化第一培养过程中的 NH4Cl、K2HPO4 和 MgSO4·7H2O 浓度时，生物量的最大浓度为 3.34 g/L，而在优化第二培养过程中的 NaCl 和谷氨酸钠浓度时，四氢嘧啶的最大浓度为 1.27 g/L。实验得到的（3.36 g 生物量/L 和 1.25 g 四氢嘧啶/L）与预测值吻合良好。优化条件还用于两步 1.5 L 补料分批发酵。在第一步中，获得了 28.7 g/L 的生物量浓度，而在第二步中，生物量浓度增加到 63 g/L。获得了 9.2 g/L 的四氢嘧啶浓度，总四氢嘧啶生产率为 6.3 g/L/天，这是迄今为止报道的最高水平之一。
