Nakagawa Hiroyuki, Terasaki Asako G, Suzuki Hiroshi, Ohashi Kazuyo, Miyamoto Shigeaki
Division of Biology, Faculty of Science, Fukuoka University, Fukuoka 814-0180, Japan.
FEBS Lett. 2006 May 29;580(13):3223-8. doi: 10.1016/j.febslet.2006.04.082. Epub 2006 May 4.
Actin filaments are organised into sub-compartments of meshwork and bundles in lamellipodia. Localisation of fascin, the LIM and SH3 domain protein 1 (lasp-1), and lasp-2 to the bundles suggest their involvement in that organisation; however, their contributions remain unclear. We have compared the turnover of these proteins with actin at the bundle. After photobleaching, EGFP-actin recovered inwards from the bundle tip, consistent with the retrograde flow by treadmilling. In contrast, the recovery of EGFP-fascin, -lasp-1 and -lasp-2 occurred from the anterograde direction. These results suggest that these molecules would participate in the stabilisation of bundles but not in initiation.
肌动蛋白丝在片足中被组织成网络和束状的亚区室。成束蛋白、LIM和SH3结构域蛋白1（LASP-1）以及LASP-2在束中的定位表明它们参与了这种组织；然而，它们的作用仍不清楚。我们比较了这些蛋白质与肌动蛋白在束处的周转情况。光漂白后，EGFP-肌动蛋白从束尖端向内恢复，这与踏车行为导致的逆行流动一致。相比之下，EGFP-成束蛋白、-LASP-1和-LASP-2的恢复是从顺行方向发生的。这些结果表明，这些分子可能参与束的稳定而非起始。
