Panteri Roger, Mey Jörg, Zhelyaznik Nina, D'Altocolle Anna, Del Fà Aurora, Gangitano Carlo, Marino Ramona, Lorenzetto Erika, Buffelli Mario, Keller Flavio
Laboratorio di Neuroscienze dello Sviluppo, Università Campus Bio-Medico, 00155 Roma, Italy.
Mol Cell Neurosci. 2006 May-Jun;32(1-2):133-42. doi: 10.1016/j.mcn.2006.03.004. Epub 2006 May 11.
Reelin is an extracellular matrix protein which is critical for the positioning of migrating post-mitotic neurons and the laminar organization of several brain structures during development. We investigated the expression and localization of Reelin in the rodent peripheral nerve during postnatal development and following crush injury in the adult stage. As shown with Western blotting, immunocytochemistry and RT-PCR, Schwann cells in the developing peripheral nerve and in primary cultures from neonatal nerves produce and secrete Reelin. While Reelin levels are downregulated in adult stages, they are again induced following sciatic nerve injury. A morphometric analysis of sciatic nerve sections of reeler mice suggests that Reelin is not essential for axonal ensheathment by Schwann cells, however, it influences the caliber of myelinated axons and the absolute number of fibers per unit area. This indicates that Reelin may play a role in peripheral nervous system development and repair by regulating Schwann cell-axon interactions.
Reelin是一种细胞外基质蛋白，在发育过程中对于有丝分裂后迁移神经元的定位以及几个脑结构的层状组织至关重要。我们研究了Reelin在出生后发育期间以及成年期挤压伤后在啮齿动物周围神经中的表达和定位。如蛋白质免疫印迹、免疫细胞化学和逆转录-聚合酶链反应所示，发育中的周围神经以及新生神经原代培养物中的施万细胞产生并分泌Reelin。虽然Reelin水平在成年期下调，但在坐骨神经损伤后又被诱导。对reeler小鼠坐骨神经切片的形态计量分析表明，Reelin对于施万细胞包裹轴突并非必不可少，然而，它会影响有髓轴突的直径以及每单位面积纤维的绝对数量。这表明Reelin可能通过调节施万细胞与轴突的相互作用在周围神经系统发育和修复中发挥作用。
