Reinders Judith, Rozemuller Erik H, van der Ven Kevin J W, Caillat-Zucman Sophie, Slootweg Pieter J, de Weger Roel A, Tilanus Marcel G J
Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands.
Hum Immunol. 2006 Mar;67(3):196-203. doi: 10.1016/j.humimm.2006.02.006. Epub 2006 Mar 29.
Many immune-related genes are located within the human leukocyte antigen (HLA) region on chromosome 6. The MHC class I chain-related gene A (MICA), located centromeric of HLA-B, is involved in the innate and adaptive immune response through activation of NK and T cells. Differences of MICA transmembrane repeat lengths have been associated with diseases and expression is observed on epithelial tumors. Head and neck squamous cell carcinoma (HNSCC) is an epithelial tumor. In the present study we evaluated the MICA repeat length diversity in relation to MICA expression in Dutch HNSCC patients. MICA short tandem repeat analysis indicated a significant decrease in the frequency for the MICA-A9 repeat in patients diagnosed with oral cavity squamous cell carcinoma (SCC) but not in patients with SCC in the hypoharynx, larynx, or oropharynx. Interestingly, the majority of patients expressed MICA as observed with immunohistochemical staining whereas no soluble MICA was detected in patients' sera by enzyme-linked immunosorbent assay. In conclusion, the length of the MICA transmembrane repeats in Dutch HNSCC patients does not influence the MICA expression on tumor cells.
许多免疫相关基因位于6号染色体上的人类白细胞抗原（HLA）区域内。MHC I类链相关基因A（MICA）位于HLA - B的着丝粒侧，通过激活自然杀伤细胞（NK）和T细胞参与先天性和适应性免疫反应。MICA跨膜重复长度的差异与疾病有关，并且在上皮肿瘤中可观察到其表达。头颈部鳞状细胞癌（HNSCC）是一种上皮肿瘤。在本研究中，我们评估了荷兰HNSCC患者中MICA重复长度多样性与MICA表达的关系。MICA短串联重复序列分析表明，诊断为口腔鳞状细胞癌（SCC）的患者中MICA - A9重复序列的频率显著降低，但下咽、喉或口咽SCC患者中未出现这种情况。有趣的是，通过免疫组织化学染色观察到大多数患者表达MICA，而通过酶联免疫吸附测定在患者血清中未检测到可溶性MICA。总之，荷兰HNSCC患者中MICA跨膜重复序列的长度不影响肿瘤细胞上的MICA表达。
