Kitcharoen K, Witt C S, Romphruk A V, Christiansen F T, Leelayuwat C
Medical Technology Program, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand.
Hum Immunol. 2006 Mar;67(3):238-46. doi: 10.1016/j.humimm.2006.02.012. Epub 2006 Mar 29.
Genetic testing of the MHC is required for selection of donors for bone marrow transplantation. The outcome of related bone marrow transplantation is usually superior to that of unrelated bone marrow transplantation. This may be the result of inaccurate or incomplete genetic testing employed for selection of donor for transplantation. Based on MHC haplotype matching, MHC block matching has been developed for selection of potential unrelated donor. Block matching has been shown previously to improve outcome when added to the conventional method of human leukocyte antigen (HLA) typing for selection of donors. In this study, we have retrospectively analyzed 44 donor recipient pairs from the Australian Bone Marrow Donor Registry Repository with respect to matching of HLA-B and HLA-Cw by sequence-based typing and MICA and MICB by polymerase chain reaction-sequence specific primer and MHC beta block matching and correlated these results with survival. Beta block matching was correlated with MIC matching (p < 0.005) and with HLA-B and HLA-Cw matching. Patients who were HLA-B and -Cw matched had significantly improved survival when they were additionally matched for MHC beta block (p(c) = 0.04) or MIC (p(c) = 0.05).
骨髓移植供体的选择需要进行MHC基因检测。亲缘性骨髓移植的结果通常优于非亲缘性骨髓移植。这可能是由于用于移植供体选择的基因检测不准确或不完整所致。基于MHC单倍型匹配，已开发出MHC模块匹配法来选择潜在的非亲缘供体。先前的研究表明，在传统的人类白细胞抗原（HLA）分型供体选择方法中加入模块匹配可改善移植结果。在本研究中，我们对澳大利亚骨髓供体登记库中的44对供体-受体进行了回顾性分析，通过基于序列的分型方法检测HLA-B和HLA-Cw的匹配情况，通过聚合酶链反应-序列特异性引物检测MICA和MICB的匹配情况以及MHCβ模块匹配情况，并将这些结果与生存率进行关联分析。β模块匹配与MIC匹配相关（p < 0.005），也与HLA-B和HLA-Cw匹配相关。HLA-B和-Cw匹配的患者在同时进行MHCβ模块匹配（p(c) = 0.04）或MIC匹配（p(c) = 0.05）时，生存率有显著提高。
