环氧化酶在高动力型门静脉高压大鼠中的表达
Expression of cyclooxygenase in hyperdynamic portal hypertensive rats.
作者信息
Cao Hui, Xu Jia, Hua Rong, Meng Fang-Bin, Qiu Jiang-Feng, Wu Zhi-Yong
机构信息
Department of Surgery, Renji Hospital, Shanghai Second Medical University, Shanghai 200127, China.
出版信息
Hepatobiliary Pancreat Dis Int. 2006 May;5(2):252-6.
BACKGROUND
By detecting hemodynamic changes, concentration of plasm prostacyclin (PGI2) and expression of cyclooxygenase (COX) in vasculature and splanchnic tissues, we evaluated the relative contributions of PGI2 and COX mRNA expression to the hyperdynamic circulatory state in chronic portal hypertensive rats.
METHODS
Fifty male Sprague-Dawley rats were divided into 3 groups: intrahepatic portal hypertension (IHPH, n=18) by injection of CCl4, prehepatic portal hypertension (PHPH, n=18) by partial stenosis of the portal vein, and sham-operated controls (SO, n=14). Splanchnic hemodynamics was measured by radioactive microsphere techniques and the concentration of PGI2 was detected by specific radioimmunoassay for its stable hydrolysis product 6-keto-PGF1alpha. Semi-quantitive reverse transcriptase-polymerase chain reaction (RT-PCR) was performed to measure the levels of COX-1 mRNA and COX-2 mRNA in the thoracic aorta, superior mesenteric artery (SMA), and small intestine of IHPH, PHPH and SO rats, respectively.
RESULTS
Hyperdynamic circulatory state was characterized by increased splanchnic blood flow and decreased splanchnic vascular resistance in IHPH and PHPH rats. The concentration of plasma 6-keto-PGF1alpha (pg/ml) in IHPH (1093.75+/-142.15) and PHPH (897.42+/-53.29) rats was significantly higher than that in SO rats (730.13+/-98.67) (P<0.05). The expression of COX-1 mRNA in the thoracic aorta, SMA and small intestine was enhanced, whereas COX-2 mRNA expression was not detected in either of these vessels or the small intestine. The plasma 6-keto-PGF1alpha concentration and the expression of COX-1 mRNA in these vessels and the small intestine were closely correlated with such hemodynamic parameters as portal venous inflow (PVI), splanchnic vascular resistance (SVR) and free portal venous pressure (FPP) (P<0.05).
CONCLUSION
The expression of COX-1 mRNA and the levels of PGI2 were closely related to the hyperdynamic circulatory state of portal hypertensive rats.
背景
通过检测血流动力学变化、血浆前列环素（PGI2）浓度以及血管和内脏组织中环氧化酶（COX）的表达，我们评估了PGI2和COX mRNA表达对慢性门静脉高压大鼠高动力循环状态的相对贡献。
方法
将50只雄性Sprague-Dawley大鼠分为3组：通过注射四氯化碳建立肝内门静脉高压（IHPH，n = 18），通过部分门静脉狭窄建立肝前门静脉高压（PHPH，n = 18），以及假手术对照组（SO，n = 14）。采用放射性微球技术测量内脏血流动力学，通过特异性放射免疫分析法检测PGI2稳定水解产物6-酮-PGF1α的浓度，以测定PGI2浓度。分别采用半定量逆转录-聚合酶链反应（RT-PCR）检测IHPH、PHPH和SO大鼠胸主动脉、肠系膜上动脉（SMA）和小肠中COX-1 mRNA和COX-2 mRNA的水平。
结果
IHPH和PHPH大鼠的高动力循环状态表现为内脏血流量增加和内脏血管阻力降低。IHPH组（1093.75±142.15）和PHPH组（897.42±53.29）大鼠血浆6-酮-PGF1α浓度（pg/ml）显著高于SO组大鼠（730.13±98.67）（P<0.05）。胸主动脉、SMA和小肠中COX-1 mRNA表达增强，而在这些血管或小肠中均未检测到COX-2 mRNA表达。这些血管和小肠中的血浆6-酮-PGF1α浓度以及COX-1 mRNA表达与门静脉流入量（PVI）、内脏血管阻力（SVR）和自由门静脉压力（FPP）等血流动力学参数密切相关（P<0.05））。
结论
COX-1 mRNA表达和PGI2水平与门静脉高压大鼠的高动力循环状态密切相关。
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