GE82832的特性研究，GE82832是一种与细菌30S核糖体亚基相互作用的转位肽抑制剂。
Characterization of GE82832, a peptide inhibitor of translocation interacting with bacterial 30S ribosomal subunits.
作者信息
Brandi Letizia, Fabbretti Attilio, Di Stefano Michele, Lazzarini Ameriga, Abbondi Monica, Gualerzi Claudio O
机构信息
Laboratory of Genetics, Department of Biology MCA, University of Camerino, Italy.
出版信息
RNA. 2006 Jul;12(7):1262-70. doi: 10.1261/rna.61206. Epub 2006 May 12.
Abstract
GE82832, a secondary metabolite produced by Streptosporangium cinnabarinum (strain GE82832), has been identified as a translational inhibitor by in vitro screening of a library of natural products. Secondary functional tests specific for individual steps of the translational pathway demonstrated that translocation is the specific target of GE82832. Chemical probing in situ demonstrated that this antibiotic protects bases A1324 and A1333 and exposes C1336 of 16S rRNA, thereby indicating that its binding site is located on the head of the 30S ribosomal subunit. The ribosomal location of GE82832, near ribosomal protein S13 and G1338, two elements of the small subunit that are part of or close to the B1a intrasubunit bridge, suggests that translocation inhibition results from an altered dynamics of 30S-50S ribosomal subunit interaction.
摘要
GE82832是由朱红链孢囊菌（菌株GE82832）产生的一种次级代谢产物，通过对天然产物文库进行体外筛选，已被鉴定为一种翻译抑制剂。针对翻译途径各个步骤的二级功能测试表明，转位是GE82832的特定作用靶点。原位化学探测表明，这种抗生素保护16S rRNA的A1324和A1333碱基，并使C1336碱基暴露，从而表明其结合位点位于30S核糖体亚基的头部。GE82832在核糖体上的位置靠近核糖体蛋白S13和G1338，这两个小亚基的元件是B1a亚基内桥的一部分或与之接近，这表明转位抑制是由30S - 50S核糖体亚基相互作用的动力学改变所致。
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