Lavaud Johann, Kroth Peter G
Group of Plant Ecophysiology, Biology Department, University of Konstanz, Germany.
Plant Cell Physiol. 2006 Jul;47(7):1010-6. doi: 10.1093/pcp/pcj058. Epub 2006 May 13.
In diatoms, the non-photochemical fluorescence quenching (NPQ) regulates photosynthesis during light fluctuations. NPQ is associated with an enzymatic xanthophyll cycle (XC) which is controlled by the light-driven transthylakoid proton gradient (delta pH). In this report, special illumination conditions and chemicals were used to perturb the kinetics of the delta pH build-up, of the XC and of NPQ. We found that the delta pH-related acidification of the lumen is also needed for NPQ to develop by switching the xanthophylls to an 'activated' state, probably via the protonation of light-harvesting antenna proteins. It confirms the NPQ model previously proposed for diatoms.
在硅藻中，非光化学荧光猝灭（NPQ）在光照波动期间调节光合作用。NPQ与一个酶促叶黄素循环（XC）相关联，该循环由光驱动的跨类囊体质子梯度（ΔpH）控制。在本报告中，使用特殊的光照条件和化学物质来干扰ΔpH积累、XC和NPQ的动力学。我们发现，通过将叶黄素切换到“激活”状态，可能是通过光捕获天线蛋白的质子化，腔室中与ΔpH相关的酸化对于NPQ的发展也是必需的。这证实了先前为硅藻提出的NPQ模型。
