Hirase Ai, Hamada Takahiro, Itoh Tomohiko J, Shimmen Teruo, Sonobe Seiji
Graduate School of Life Science, University of Hyogo, Japan.
Plant Cell Physiol. 2006 Jul;47(7):1004-9. doi: 10.1093/pcp/pcj055. Epub 2006 May 13.
The effects of butanol on microtubules (MTs) were examined by immunofluorescence microscopy. Fragmentation of cortical MTs was induced by n-butanol, but not by s- and t-butanols, in cultured tobacco BY-2 cells. Taxol prevented n-butanol-induced MT fragmentation. Fragmented cortical MTs were still attached to the inner face of the plasma membrane when n-butanol-treated protoplasts were ruptured on the slide glass. Moreover, MTs were depolymerized in the presence of n-butanol in vitro. Therefore, n-butanol is not only an activator of phospholipase D but also an effective MT-depolymerizing agent.
通过免疫荧光显微镜检查了丁醇对微管（MTs）的影响。在培养的烟草BY-2细胞中，正丁醇可诱导皮层微管断裂，而异丁醇和叔丁醇则不能。紫杉醇可防止正丁醇诱导的微管断裂。当用正丁醇处理的原生质体在载玻片上破裂时，断裂的皮层微管仍附着在质膜的内表面。此外，在体外，微管在正丁醇存在的情况下会解聚。因此，正丁醇不仅是磷脂酶D的激活剂，也是一种有效的微管解聚剂。
