Sakabe K, Sasaki K, Watanabe N, Suzuki M, Wang Z G, Miyahara J, Sakabe N
Department of Chemistry, Faculty of Science, Nagoya University, Nagoya 464, Japan.
J Synchrotron Radiat. 1997 May 1;4(Pt 3):136-46. doi: 10.1107/S0909049597003683.
Off-line and on-line protein data-collection systems using an imaging plate as a detector are described and their components reported. The off-line scanner IPR4080 was developed for a large-format imaging plate ;BASIII' of dimensions 400 x 400 mm and 400 x 800 mm. The characteristics of this scanner are a dynamic range of 10(5) photons pixel(-1), low background noise and high sensitivity. A means of reducing electronic noise and a method for finding the origin of the noise are discussed in detail. A dedicated screenless Weissenberg camera matching IPR4080 with synchrotron radiation was developed and installed on beamline BL6B at the Photon Factory. This camera can attach one or two sheets of 400 x 800 mm large-format imaging plate inside the film cassette by evacuation. The positional reproducibility of the imaging plate on the cassette is so good that the data can be processed by batch job. Data of 93% completeness up to 1.6 A resolution were collected on a single axis rotation and the value of R(merge) becomes 4% from a tetragonal lysozyme crystal using a set of two imaging-plate sheets. Comparing two types of imaging plates, the signal-to-noise ratio of the ST-VIP-type imaging plate is 25% better than that of the BASIII-type imaging plate for protein data collection using 1.0 and 0.7 A X-rays. A new on-line protein data-collection system with imaging plates is specially designed to use synchrotron radiation X-rays at maximum efficiency.
描述了使用成像板作为探测器的离线和在线蛋白质数据收集系统，并报告了其组件。离线扫描仪IPR4080是为大幅面成像板开发的；尺寸为400×400mm和400×800mm的“BASIII”。该扫描仪的特点是动态范围为10(5)光子像素(-1)，背景噪声低且灵敏度高。详细讨论了降低电子噪声的方法和寻找噪声来源的方法。开发了一种与同步辐射相匹配的专用无屏魏森堡相机IPR4080，并安装在光子工厂的BL6B光束线上。该相机可通过抽真空在暗盒内安装一张或两张400×800mm的大幅面成像板。成像板在暗盒上的位置再现性非常好，数据可以通过批处理作业进行处理。使用一组两张成像板，从四方溶菌酶晶体在单轴旋转上收集了高达1.6 Å分辨率的93%完整性的数据，R(merge)值变为4%。比较两种类型的成像板，对于使用1.0 Å和0.7 Å X射线进行蛋白质数据收集，ST-VIP型成像板的信噪比比BASIII型成像板高25%。一种新的带有成像板的在线蛋白质数据收集系统经过专门设计，以最大效率使用同步辐射X射线。
