Kamiya N, Sasaki K, Watanabe N, Sakabe N, Sakabe K
Institute of Physical and Chemical Research (RIKEN), Hirosawa 2-1, Wako 351-01, Japan.
J Synchrotron Radiat. 1997 Jan 1;4(Pt 1):14-6. doi: 10.1107/S0909049596013829.
A diffraction-intensity data-collection system with synchrotron radiation X-rays utilizing the screenless Weissenberg technique and incorporating a large-format imaging plate is one of the most suitable apparatus for time-resolved protein crystallography with larger angle oscillations than hitherto described. The time resolution and data quality of the system have been tested using a tetragonal lysozyme crystal as a test sample in a flow-cell experiment at the bending-magnet beamline 18B at the Photon Factory, and a time resolution of 15 min is confirmed.
一种利用无屏魏森堡技术并配备大幅面成像板的同步辐射X射线衍射强度数据收集系统，是用于时间分辨蛋白质晶体学且角度振荡比迄今所描述的更大的最合适仪器之一。该系统的时间分辨率和数据质量已在光子工厂弯曲磁铁光束线18B的流动池实验中，使用四方溶菌酶晶体作为测试样品进行了测试，并确认时间分辨率为15分钟。
