Inouye Shigeharu, Uchida Katsuhisa, Maruyama Naho, Yamaguchi Hideyo, Abe Shigeru
Teikyo University Institute of Medical Mycology, Tokyo, Japan.
Nihon Ishinkin Gakkai Zasshi. 2006;47(2):91-8. doi: 10.3314/jjmm.47.91.
By the combined use of agar diffusion, agar vapor and agar vapor-inhibitory assays, contribution of the vapor activity of essential oils was quantitatively estimated. The test organisms were Trichophyton mentagrophytes and Aspergillus fumigatus. Agar vapor assay was used to confirm the vapor activity of the oils. The parameter delta defined as a contribution index of the vapor activity was calculated by (1 - b-c/a-c) x 100, where a is inhibitory diameter in the diffusion assay, b is inhibitory diameter in the vapor-inhibitory assay and c is diameter of the sealed ring in the vapor-inhibitory assay (21 mm). Many of the essential oils examined showed a delta value near 100, thus providing the major contribution of the vapor activity to the inhibitory diameter. Essential oils containing aldehyde as major constituent showed low delta value, indicating the major inhibition was due to agar diffusion. Major essential oil components behaved similarly; the delta value was increased in the following order: aldehyde < phenol < alcohol < ester, oxide, hydrocarbon, indicating the enhanced contribution of the vapor activity in that order.
通过联合使用琼脂扩散法、琼脂蒸汽法和琼脂蒸汽抑制法，对香精油蒸汽活性的贡献进行了定量评估。测试微生物为须癣毛癣菌和烟曲霉。琼脂蒸汽法用于确认香精油的蒸汽活性。定义为蒸汽活性贡献指数的参数δ通过(1 - b - c/a - c)×100计算得出，其中a为扩散试验中的抑菌直径，b为蒸汽抑制试验中的抑菌直径，c为蒸汽抑制试验中密封环的直径(21毫米)。许多被检测的香精油显示δ值接近100，因此表明蒸汽活性对抑菌直径的贡献很大。以醛为主要成分的香精油显示出较低的δ值，表明主要抑制作用是由于琼脂扩散。主要香精油成分表现类似；δ值按以下顺序增加：醛＜酚＜醇＜酯、氧化物、烃，表明蒸汽活性的贡献按此顺序增强。
