Leruez-Ville M, Chardin-Ouachée M, Neven B, Picard C, Le Guinche I, Fischer A, Rouzioux C, Blanche S
Laboratoire de Virologie, EA 3620 Université Paris V, Hôpital Necker-Enfants Malades AP-HP, Paris, France.
Bone Marrow Transplant. 2006 Jul;38(1):23-8. doi: 10.1038/sj.bmt.1705389. Epub 2006 May 15.
Adenovirus infections result in significant morbidity and mortality in allogeneic haematopoietic stem cell transplanted (hSCT) children. Adenovirus from species C and B account for more than 90% of adenoviruses recovered after hSCT. However, infections due to adenovirus A31 have been increasingly reported in recent years. Between April 2002 and April 2005, blood samples obtained every 2 weeks from 58 hSCT children were screened for adenovirus species A to C by quantitative real-time PCR. Phylogenetic analysis was realized after amplification and sequencing of the entire hexon gene. Fifteen cases of adenovirus infection with viraemia were recovered during this 3 years period. During spring/summer 2003, seven cases occurred and were due to an adenovirus species A. Phylogenetic analysis of the seven strains showed that they belonged to the A31 genotype and shared 100% homology. Clinical features of the seven HSCT children with A31 adenovirus viraemia are described. We describe here an epidemic spread of adenovirus genotype A31 in a paediatric haematology unit. Timing, location and hexon gene genotyping results highly suggested a nosocomial origin to this epidemic. The burden of adenovirus A31 infection needs to be further assessed in this context.
腺病毒感染在异基因造血干细胞移植（hSCT）儿童中会导致显著的发病率和死亡率。C 种和 B 种腺病毒占 hSCT 后回收的腺病毒的 90%以上。然而，近年来 A31 型腺病毒感染的报道越来越多。在 2002 年 4 月至 2005 年 4 月期间，通过定量实时 PCR 对 58 名 hSCT 儿童每 2 周采集的血样进行 A 至 C 种腺病毒筛查。在对整个六邻体基因进行扩增和测序后进行系统发育分析。在这 3 年期间共发现 15 例腺病毒血症感染病例。在 2003 年春/夏，发生了 7 例病例，均由 A 种腺病毒引起。对这 7 株病毒的系统发育分析表明，它们属于 A31 基因型，同源性为 100%。描述了 7 例患有 A31 型腺病毒血症的 hSCT 儿童的临床特征。我们在此描述了 A31 型腺病毒在一家儿科血液科病房的流行传播情况。时间、地点和六邻体基因分型结果强烈表明此次疫情起源于医院感染。在这种情况下，A31 型腺病毒感染的负担需要进一步评估。
