在日本引起医院内流行性角结膜炎感染的19型腺病毒新基因组类型。

New genome type of adenovirus serotype 19 causing nosocomial infections of epidemic keratoconjunctivitis in Japan.

作者信息

Tanaka-Yokogui K, Itoh N, Usui N, Takeuchi S, Uchio E, Aoki K, Usui M, Ohno S

机构信息

Department of Ophthalmology, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan.

出版信息

J Med Virol. 2001 Nov;65(3):530-3.

PMID:11596089

Abstract

Twelve strains of adenovirus serotype 19, isolated from cases of epidemic keratoconjunctivitis in Japan in 1992, 1993, 1997, and 1998, were analyzed by DNA restriction analysis, using restriction endonucleases BamHI, BglI, BglII, EcoRI, HindIII, KpnI, PstI, SacI, SalI, SmaI, and XhoI. Among these 11 restriction endonucleases, EcoRI, PstI, SacI, and SmaI were discriminative enzymes, showing restriction patterns different from those reported previously for the prototype and the variant 19a. This new genome type was isolated in 1997 and 1998, when an increase of epidemic keratoconjunctivitis cases caused by adenovirus serotype 19 was observed for both sporadic and nosocomial infections. Strains from 1992 and 1993 showed restriction patterns similar to those of the worldwide reported variant 19a for all enzymes used. The changes detected in strains from 1997 and 1998 could be the reason for the recent epidemic.

摘要

对1992年、1993年、1997年和1998年从日本流行性角结膜炎病例中分离出的12株19型腺病毒，使用限制性内切酶BamHI、BglI、BglII、EcoRI、HindIII、KpnI、PstI、SacI、SalI、SmaI和XhoI进行DNA限制性分析。在这11种限制性内切酶中，EcoRI、PstI、SacI和SmaI是鉴别性酶，其显示出与先前报道的原型和19a变体不同的限制性图谱。这种新的基因组类型于1997年和1998年分离得到，当时观察到由19型腺病毒引起的流行性角结膜炎病例在散发性和医院感染中均有所增加。1992年和1993年的菌株对所有使用的酶显示出与全球报道的19a变体相似的限制性图谱。1997年和1998年菌株中检测到的变化可能是近期疫情的原因。