新型隐球菌加蒂变种、格鲁比变种和新型变种中的超氧化物歧化酶

Superoxide dismutase in Cryptococcus neoformans varieties gattii, grubi, and neoformans.

作者信息

Dias A L T, Brigagão M R P L, Colepicolo P, Siqueira A M, Silva E G da, Paula C R

机构信息

Departamento de Microbiologia, Instituto de Ciências Biomédicas II, São Paulo, SP, Brazil.

出版信息

Mem Inst Oswaldo Cruz. 2006 Feb;101(1):107-9. doi: 10.1590/s0074-02762006000100021. Epub 2006 May 12.

DOI:10.1590/s0074-02762006000100021

PMID:16699719

Abstract

Some clear dissimilarities occur among the varieties of Cryptococcus neoformans but there are few studies about the differences among individual yeast antioxidant enzymes. The total superoxide dismutase (SOD) activities and the copper, zinc-depend SOD (Cu,ZnSOD) and manganese-dependent SOD (MnSOD) isoenzymes of five reference C. neoformans strains belonged to A, B, C, AD and D serotypes (Table I) and other nine C. neoformans isolates (Table II) were determined. There were significant differences (p < 0.01 and p < 0.05) in total SOD activity among the varietie gattii (serotype C) and the other varieties. Cu,ZnSOD showed difference (p < 0.05) between A and D serotypes. These results point out a variety and serotype-independent SOD activity in C. neoformans reference strains and the other isolates that were evaluated.

摘要

新型隐球菌的不同变种之间存在一些明显差异，但关于单个酵母抗氧化酶之间差异的研究较少。测定了属于A、B、C、AD和D血清型的5株新型隐球菌参考菌株（表I）以及其他9株新型隐球菌分离株（表II）的总超氧化物歧化酶（SOD）活性以及铜锌依赖性SOD（Cu,ZnSOD）和锰依赖性SOD（MnSOD）同工酶。在加蒂变种（血清型C）和其他变种之间，总SOD活性存在显著差异（p < 0.01和p < 0.05）。Cu,ZnSOD在A和D血清型之间存在差异（p < 0.05）。这些结果表明，在被评估的新型隐球菌参考菌株和其他分离株中，SOD活性与变种和血清型无关。