Nishimura M, Hara A, Nojima H, Noda S, Mashimo M, Hori Y
Department of Dermatology, Kyushu University, Beppu, Japan.
Sarcoidosis. 1991 Sep;8(2):101-4.
We studied effects of the hormonal form of vitamin D3 on the angiotensin-converting enzyme (ACE) activity of hepatic granulomas in mice infected with Schistosoma mansoni. During 7 to 11 weeks after infection, mice were given orally 0.1 or 1.0 microgram/kg of 1 alpha (OH)D3 or only medium solution every other day. Granulomatous livers were removed at 7, 9 and 11 weeks after infection, and ACE activity was measured in the granulomas isolated from each liver tissue using a fluorometric method. Oral administration of 0.1 or 1.0 microgram/kg/2 days of 1 alpha (OH)D3 for 4 weeks significantly enhanced ACE activity in the granuloma tissue. Since the DNA content relative to a unit weight of protein in the granulomas did not change with the 1 alpha (OH)D3 treatment, it is suggested that the elevated tissue ACE activity is due to an actual increase of the enzyme activity in each granuloma cell. The present observation may have relevance to sarcoid granulomas characterized by an increased tissue ACE activity, since macrophages from patients with sarcoidosis synthesize a biologically active hormonal form of vitamin D3. Namely hormonal form of vitamin D3 locally produced by macrophages is involved not only in systemic Ca++ metabolism but also in the stimulation of macrophages themselves to produce ACE in the granulomas.
我们研究了维生素D3的激素形式对感染曼氏血吸虫的小鼠肝脏肉芽肿中血管紧张素转换酶（ACE）活性的影响。在感染后的7至11周内，每隔一天给小鼠口服0.1或1.0微克/千克的1α（OH）D3或仅给予培养基溶液。在感染后的第7、9和11周取出肉芽肿性肝脏，使用荧光法测量从每个肝脏组织分离出的肉芽肿中的ACE活性。连续4周每隔一天口服0.1或1.0微克/千克/2天的1α（OH）D3可显著提高肉芽肿组织中的ACE活性。由于肉芽肿中相对于单位重量蛋白质的DNA含量在1α（OH）D3处理后没有变化，提示组织ACE活性升高是由于每个肉芽肿细胞中酶活性的实际增加。本观察结果可能与以组织ACE活性增加为特征的结节病肉芽肿有关，因为结节病患者的巨噬细胞可合成具有生物活性的维生素D3激素形式。也就是说，巨噬细胞局部产生的维生素D3激素形式不仅参与全身钙代谢，还参与刺激巨噬细胞自身在肉芽肿中产生ACE。
