Pantaleo V, Grieco F, Di Franco A, Martelli G P
Istituto di Virologia Vegetale del CNR, Sezione di Bari and Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università degli Studi, Bari, Italy.
Arch Virol. 2006 Oct;151(10):1973-83. doi: 10.1007/s00705-006-0767-2. Epub 2006 May 15.
A full-length cDNA clone of olive latent virus 1 (OLV-1), a member of the genus Necrovirus, family Tombusviridae, was subjected to site-directed mutagenesis, and coat protein gene mutants were constructed. A mutant clone, denoted Delta3297, was obtained by deleting the nucleotide in position 3297, thus inducing a frameshift and replacing the last 49 amino acids of the viral coat protein (CP) by a shorter sequence of 39 amino acids. This mutant was viable, stable, able to synthesize a smaller CP, and able to give rise to the formation of apparently intact virus particles. Cell-to-cell movement of Delta3297 in Nicotiana benthamiana leaves was not affected, but, contrary to wild type OLV-1, it failed to spread systemically. These results indicate that virion formation is necessary but not sufficient for long-distance movement for OLV-1 and highlights the role of the CP carboxy-terminal domain in systemic infection.
对番茄丛矮病毒科坏死病毒属成员油橄榄潜隐病毒1（OLV-1）的全长cDNA克隆进行了定点诱变，并构建了外壳蛋白基因突变体。通过缺失第3297位核苷酸获得了一个名为Delta3297的突变体克隆，从而导致移码，并使病毒外壳蛋白（CP）的最后49个氨基酸被39个氨基酸的较短序列取代。该突变体是有活力的、稳定的，能够合成较小的CP，并能够产生明显完整的病毒粒子。Delta3297在本氏烟草叶片中的细胞间移动不受影响，但与野生型OLV-1相反，它不能进行系统传播。这些结果表明，病毒粒子的形成对于OLV-1的长距离移动是必要的，但不是充分的，并突出了CP羧基末端结构域在系统感染中的作用。
