正常渗透压和低渗透压大鼠视上核基因表达的微阵列分析。
Microarray analysis of gene expression in the supraoptic nucleus of normoosmotic and hypoosmotic rats.
作者信息
Yue Chunmei, Mutsuga Noriko, Verbalis Joseph, Gainer Harold
机构信息
Laboratory of Neurochemistry, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
出版信息
Cell Mol Neurobiol. 2006 Jul-Aug;26(4-6):959-78. doi: 10.1007/s10571-006-9017-0. Epub 2006 May 13.
Abstract
- Hypoosmolality produces a dramatic inhibition of vasopressin (VP) and oxytocin (OT) gene expression in the supraoptic nucleus (SON). This study examines the effect of sustained hypoosmolality on global gene expression in the OT and VP magnocellular neurons (MCNs) of the hypothalamo-neurohypophysial system (HNS), in order to detect novel genes in this system that might be involved in osmoregulation in the MCNs. 2. For this purpose, we used Affymetrix oligonucleotide arrays to analyze the expression of specific genes in laser microdissected rat SONs, and their changes in expression during chronic hypoosmolality. We identified over 40 genes that had three-fold or more greater expression in the SON versus total hypothalamus, and that also changed more than two fold in expression as a result of the chronic hypoosmolar treatment. These genes contained both novel as well as genes previously known to be present in the SON. All of the raw data for the genes that are expressed in the SON and altered by hypoosmolality can be found on the following NINDS website URL address: http://data.ninds.nih.gov/Gainer/Publications.
摘要
- 低渗状态会对视上核（SON）中血管加压素（VP）和催产素（OT）的基因表达产生显著抑制作用。本研究旨在探究持续低渗状态对下丘脑 - 神经垂体系统（HNS）中OT和VP大细胞神经元（MCNs）整体基因表达的影响，以检测该系统中可能参与MCNs渗透压调节的新基因。2. 为此，我们使用Affymetrix寡核苷酸阵列分析激光显微切割的大鼠视上核中特定基因的表达情况，以及慢性低渗状态下它们的表达变化。我们鉴定出40多个基因，这些基因在视上核中的表达比整个下丘脑高出三倍或更多，并且由于慢性低渗处理，其表达变化超过两倍。这些基因既包括新基因，也包括先前已知存在于视上核中的基因。所有在视上核中表达且因低渗状态而改变的基因的原始数据可在以下美国国立神经疾病和中风研究所（NINDS）网站的URL地址找到：http://data.ninds.nih.gov/Gainer/Publications 。
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