Glasgow Eric, Ryu Seung-Lim, Yamashita Mitsuo, Zhang Bing-Jun, Mutsuga Noriko, Gainer Harold
Department of Neurobiology, Northeastern Ohio Universities College of Medicine, Rootstown, OH 44272, USA.
Brain Res Mol Brain Res. 2005 Jun 13;137(1-2):143-51. doi: 10.1016/j.molbrainres.2005.02.030. Epub 2005 Apr 8.
Vasopressin (VP) and oxytocin (OT) play critical roles in the regulation of salt and water balance, lactation, and various behaviors and are expressed at very high levels in specific magnocellular neurons (MCNs) in the hypothalamo-neurohypophysial system (HNS). In addition to the cell-specific expression of the VP and OT genes in these cells, there are other transcripts that are preferentially expressed in the VP or OT MCNs. One such gene, paternally expressed gene 3 (Peg3), is an imprinted gene expressed exclusively from the paternal allele that encodes a Kruppel-type zinc finger-containing protein involved in maternal behavior and is abundantly expressed in the VP-MCNs. We report here the robust expression in the VP-MCNs of an RNA, which we designate APeg3 that is transcribed in the antisense direction to the 3' untranslated region of the Peg3 gene. The APeg3 mRNA is about 1 kb in size, and the full-length sequence of APeg3, as determined by 5' and 3' RACE, contains an open reading frame that predicts a protein of 93 amino acids and is predominantly expressed in VP-MCNs. Both Peg3 and APeg3 gene expression in the VP-MCNs increase during systemic hyperosmolality in vivo, demonstrating that both of these genes are osmoregulated.
血管加压素（VP）和催产素（OT）在盐和水平衡、泌乳以及各种行为的调节中发挥着关键作用，并且在下丘脑 - 神经垂体系统（HNS）中的特定大细胞神经元（MCNs）中高表达。除了这些细胞中VP和OT基因的细胞特异性表达外，还有其他转录本在VP或OT MCNs中优先表达。其中一个这样的基因，父系表达基因3（Peg3），是一个印记基因，仅从父本等位基因表达，编码一种参与母性行为的含Kruppel型锌指蛋白，并且在VP - MCNs中大量表达。我们在此报告一种RNA在VP - MCNs中的强烈表达，我们将其命名为APeg3，它以反义方向转录至Peg3基因的3'非翻译区。APeg3 mRNA大小约为1 kb，通过5'和3' RACE确定的APeg3全长序列包含一个开放阅读框，预测编码一个93个氨基酸的蛋白质，并且主要在VP - MCNs中表达。体内全身高渗状态下，VP - MCNs中Peg3和APeg3基因表达均增加，表明这两个基因均受渗透压调节。
