Bandyopadhyay Krisanu, Tan Eric, Ho Lin, Bundick Sarah, Baker Shenda M, Niemz Angelika
Keck Graduate Institute, Claremont, California 91711, USA.
Langmuir. 2006 May 23;22(11):4978-84. doi: 10.1021/la0534773.
We report the deposition of DNA-conjugated gold nanospheres into arrays of surface nanopores obtained from hexagonally ordered thin polystyrene-b-poly(methyl methacrylate) (PS-b-PMMA) diblock copolymer films on silicon. The deposition occurs spontaneously from aqueous solution and is driven by either electrostatic interactions or specific DNA hybridization events between the DNA nanospheres and the surface nanopores. To mitigate this spontaneous deposition, we have chemically modified the nanopores with either positively charged aminosilanes or oligonucleotide probe sequences. The deposition of DNA nanospheres into the surface nanopores was characterized by atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS). We have observed preferential immobilization of individual DNA nanospheres within the nanopores, based on the size matching between the two entities. The inclusion density and selectivity of DNA nanosphere deposition into the surface nanopores was found to depend predominantly on the methods through which the nanoporous surfaces were prepared and chemically functionalized.
我们报道了将DNA共轭金纳米球沉积到由硅上的六方有序聚苯乙烯-b-聚（甲基丙烯酸甲酯）（PS-b-PMMA）二嵌段共聚物薄膜获得的表面纳米孔阵列中。沉积过程从水溶液中自发发生，由DNA纳米球与表面纳米孔之间的静电相互作用或特定的DNA杂交事件驱动。为了减轻这种自发沉积，我们用带正电荷的氨基硅烷或寡核苷酸探针序列对纳米孔进行了化学修饰。通过原子力显微镜（AFM）和X射线光电子能谱（XPS）对DNA纳米球在表面纳米孔中的沉积进行了表征。基于两者之间的尺寸匹配，我们观察到单个DNA纳米球优先固定在纳米孔内。发现DNA纳米球沉积到表面纳米孔中的包含密度和选择性主要取决于制备纳米多孔表面和进行化学功能化的方法。
