Rubinoff Daniel, Cameron Stephen, Will Kipling
Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI 96822, USA.
Trends Ecol Evol. 2006 Jan;21(1):1-2. doi: 10.1016/j.tree.2005.10.019. Epub 2005 Nov 14.
In a recent study, Kress et al. compared two plant genomes to seek out plant DNA barcodes. Two promising markers balanced the variability that is needed to distinguish species with conserved primer regions that enable universal amplification. Although this study is the most rigorous effort to date, problems from earlier barcoding efforts, such as the use of non-evolutionary species concepts and differential sorting of genes and species, could reemerge. Single-gene barcoding might not be universally effective owing to inherent inaccuracies. Kress et al. suggest the use of multiple genes, reflecting an integrated approach that is likely to be the best answer to identifying species quickly and accurately.
在最近的一项研究中，克雷斯等人比较了两种植物基因组以寻找植物DNA条形码。两个有前景的标记平衡了区分物种所需的变异性与能够实现通用扩增的保守引物区域。尽管这项研究是迄今为止最严谨的工作，但早期条形码研究中的问题，如使用非进化物种概念以及基因和物种的差异分类，可能会再次出现。由于固有的不准确性，单基因条形码可能并非普遍有效。克雷斯等人建议使用多个基因，这反映了一种综合方法，可能是快速准确识别物种的最佳答案。
