Thabet Olfa Ben Dhia, Fardeau Marie-Laure, Joulian Catherine, Thomas Pierre, Hamdi Moktar, Garcia Jean-Louis, Ollivier Bernard
Laboratoire de Microbiologie IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France.
Anaerobe. 2004 Jun;10(3):185-90. doi: 10.1016/j.anaerobe.2004.04.002.
A new sporulated fermentative bacterium designated strain E1(T) (T=type strain), was isolated from an anaerobic mud of an olive mill wastewater basin contaminated by phosphogypse produced by a Tunisian factory. Strain E1(T) was a motile Gram-positive slightly curved rod with spherical terminal spore swelling the cell. It grew between 18 degrees C and 43 degrees C with an optimum at 37 degrees C and pH 7.8 (range 5.5-8.7), without NaCl (range 0-3%). Strain E1(T) was a chemoorganotrophic anaerobic bacterium fermenting only proteins and amino acids. Yeast extract was required for growth. Elemental sulfur was used as terminal electron acceptor. The G+C content of the DNA was 32.6 mol%. The closest phylogenetical relatives of strain E1(T) were Clostridium thiosulfatireducens and C. subterminale (97.3% similarity for partial rRNA gene sequences). DNA-DNA hybridization values between strain E1(T) and both species were 17% and 20.8%, respectively. On the basis of differences in genotypic and phenotypic characteristics, strain E1(T) (DSM 15206(T), CIP 107666(T)) is proposed as the type strain of a new species, C. tunisiense sp. nov. GenBank accession number for the 16S rRNA gene sequence of strain E1(T) is AY187622.
从突尼斯一家工厂产生的磷石膏污染的橄榄榨油厂废水池厌氧污泥中分离出一种新的芽孢形成发酵细菌，命名为菌株E1(T)（T=模式菌株）。菌株E1(T)是一种运动性革兰氏阳性稍弯曲杆菌，具球形末端芽孢，使细胞膨胀。其生长温度范围为18℃至43℃，最适温度为37℃，pH值为7.8（范围5.5 - 8.7），无需NaCl（范围0 - 3%）。菌株E1(T)是一种仅发酵蛋白质和氨基酸的化能有机营养型厌氧菌。生长需要酵母提取物。元素硫用作末端电子受体。DNA的G+C含量为32.6 mol%。菌株E1(T)最接近的系统发育亲缘种是硫代硫酸盐还原梭菌和近末端梭菌（部分rRNA基因序列相似性为97.3%）。菌株E1(T)与这两个种之间的DNA - DNA杂交值分别为17%和20.8%。基于基因型和表型特征的差异，菌株E1(T)（DSM 15206(T)，CIP 107666(T)）被提议作为一个新种——突尼斯梭菌（Clostridium tunisiense sp. nov.）的模式菌株。菌株E1(T)的16S rRNA基因序列的GenBank登录号为AY187622。
