Farkas Milán, Szoboszlay Sándor, Benedek Tibor, Révész Fruzsina, Veres Péter Gábor, Kriszt Balázs, Táncsics András
Department of Environmental Safety and Ecotoxicology, Szent István University, Gödöllő, Hungary.
Regional University Center of Excellence in Environmental Industry, Szent István University, Gödöllő, Hungary.
Folia Microbiol (Praha). 2017 Jan;62(1):63-71. doi: 10.1007/s12223-016-0473-8. Epub 2016 Sep 28.
Dissimilatory iron-reducing bacteria are commonly found in microbial communities of aromatic hydrocarbon-contaminated subsurface environments where they often play key role in the degradation of the contaminants. The Siklós benzene, toluene, ethylbenzene, and xylene (BTEX)-contaminated area is one of the best characterized petroleum hydrocarbon-contaminated sites of Hungary. Continuous monitoring of the microbial community in the center of the contaminant plume indicated the presence of an emerging Geobacter population and a Rhodoferax phylotype highly associated with aromatic hydrocarbon-contaminated subsurface environments. The aim of the present study was to make an initial effort to enrich Rhodoferax-related and other dissimilatory iron-reducing bacteria from this environment. Accordingly, four slightly different freshwater media were used to enrich Fe(III) reducers, differing only in the form of nitrogen source (organic, inorganic nitrogen or gaseous headspace nitrogen). Although enrichment of the desired Rhodoferax phylotype was not succeeded, Geobacter-related bacteria were readily enriched. Moreover, the different nitrogen sources caused the enrichment of different Geobacter species. Investigation of the diversity of benzylsuccinate synthase gene both in the enrichments and in the initial groundwater sample indicated that the Geobacter population in the center of the contaminant plume may not play a significant role in the anaerobic degradation of toluene.
异化铁还原菌常见于芳烃污染的地下环境的微生物群落中，它们在这些污染物的降解过程中通常起着关键作用。匈牙利的锡克洛什苯、甲苯、乙苯和二甲苯（BTEX）污染区域是该国特征最为明显的石油烃污染场地之一。对污染物羽流中心微生物群落的持续监测表明，存在一个新兴的地杆菌种群和一种与芳烃污染的地下环境高度相关的红育菌系统型。本研究的目的是初步尝试从该环境中富集与红育菌相关的以及其他异化铁还原菌。因此，使用了四种略有不同的淡水培养基来富集铁（III）还原菌，它们仅在氮源形式（有机氮、无机氮或气态顶空氮）上有所不同。尽管未能成功富集到所需的红育菌系统型，但与地杆菌相关的细菌很容易被富集。此外，不同的氮源导致了不同地杆菌物种的富集。对富集物和初始地下水样本中苄基琥珀酸合酶基因多样性的研究表明，污染物羽流中心的地杆菌种群可能在甲苯的厌氧降解中不起重要作用。
