新型生物素化可生物降解聚（乙二醇）-b-聚（碳酸酯-乳酸）共聚物的合成与表征

Synthesis and characterization of novel biotinylated biodegradable poly(ethylene glycol)-b-poly(carbonate-lactic acid) copolymers.

作者信息

Xie Zhigang, Guan Huili, Lü Changhai, Chen Xuesi, Jing Xiabin

机构信息

State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.

出版信息

Acta Biomater. 2005 Nov;1(6):635-41. doi: 10.1016/j.actbio.2005.07.004. Epub 2005 Sep 23.

DOI:10.1016/j.actbio.2005.07.004

PMID:16701844

Abstract

Poly(ethylene glycol)-b-poly(5-benzyloxy-trimethylene carbonate-lactic acid) copolymers (PEG-b-P(BTMC-LA)) were synthesized by ring-opening polymerization of lactide and 5-benzyloxy trimethylene carbonate in the presence of mono-hydroxyl poly(ethylene glycol) with diethyl zinc as catalyst. They were further converted into deprotected copolymers with the pendant hydroxyl groups by hydrogenolysis in the presence of Pd(OH)2/C, and finally conjugated with biotin through the free hydroxyl groups. Gel permeation chromatography, Fourier transform infrared, differential scanning calorimetry and 1H nuclear magnetic resonance studies confirmed the copolymer structures and successful attachment of biotin to the copolymer.

摘要

聚（乙二醇）-b-聚（5-苄氧基-三亚甲基碳酸酯-乳酸）共聚物（PEG-b-P（BTMC-LA））通过在单羟基聚（乙二醇）存在下，以二乙基锌为催化剂，使丙交酯和5-苄氧基三亚甲基碳酸酯进行开环聚合反应合成。在Pd(OH)₂/C存在下通过氢解将它们进一步转化为带有侧链羟基的脱保护共聚物，最后通过游离羟基与生物素缀合。凝胶渗透色谱法、傅里叶变换红外光谱法、差示扫描量热法和¹H核磁共振研究证实了共聚物的结构以及生物素与共聚物的成功连接。