Foppiano Silvia, Marshall Sally J, Saiz Eduardo, Tomsia Antoni P, Marshall Grayson W
Preventive and Restorative Dental Sciences, Division of Biomaterials and Bioengineering, University of California San Francisco, 707 Parnassus Ave., San Francisco, CA 94143, USA.
Acta Biomater. 2006 Mar;2(2):133-42. doi: 10.1016/j.actbio.2005.12.003. Epub 2006 Feb 3.
This work sought to provide a basic protocol for treatment of functionally graded bioactive glass coatings (FGC) that reliably adhere to titanium alloy (Ti6Al4V) prior to in vivo evaluation. The effect of the fabrication process on glass structure and reproducibility of the coating's properties, and the effect of cell culture conditions on the integrity of the coating were assessed. The structure of FGCs was compared to that of the as cast glass used as a top coating. X-ray diffraction (XRD) showed that the fabrication process resulted in 5.9+/-3.0 vol.% crystallization, while glass as cast was amorphous. Glass as cast and coatings behaved similarly in simulated body fluid (SBF): an amorphous layer rich in phosphate formed, and it crystallized, over 4 weeks, into apatite-like mineral (Fourier transform infrared spectroscopy (FTIR), XRD, scanning electron microscopy (SEM)). Reproducibility of the fabrication process was tested from three batches of coatings by measuring thickness and crystallinity. MC3T3-E1.4 mouse pre-osteoblast cells were cultured and induced to mineralize on FGCs, either as made or pre-conditioned in SBF. The sub-surface glass silica network in FGCs was compromised by cell culture conditions. A crystalline phosphate was formed during pre-conditioning (XRD, FTIR, and SEM). SBF-pre-conditioning stabilized the coatings. Thus incubation in SBF is recommended to produce a stable coating.
本研究旨在提供一种功能梯度生物活性玻璃涂层（FGC）的基本处理方案，该涂层在进行体内评估之前能可靠地附着于钛合金（Ti6Al4V）。评估了制备工艺对玻璃结构和涂层性能再现性的影响，以及细胞培养条件对涂层完整性的影响。将功能梯度生物活性玻璃涂层的结构与用作顶层涂层的铸态玻璃的结构进行了比较。X射线衍射（XRD）表明，制备工艺导致5.9±3.0体积%的结晶，而铸态玻璃为非晶态。铸态玻璃和涂层在模拟体液（SBF）中的行为相似：形成了富含磷酸盐的非晶层，并在4周内结晶为类磷灰石矿物（傅里叶变换红外光谱（FTIR）、XRD、扫描电子显微镜（SEM））。通过测量厚度和结晶度，对三批涂层的制备工艺再现性进行了测试。将MC3T3-E1.4小鼠前成骨细胞培养在功能梯度生物活性玻璃涂层上，并诱导其矿化，涂层可为制备好的涂层或在模拟体液中预处理过的涂层。功能梯度生物活性玻璃涂层中的亚表面玻璃二氧化硅网络因细胞培养条件而受损。预处理过程中形成了结晶磷酸盐（XRD、FTIR和SEM）。模拟体液预处理使涂层稳定。因此，建议在模拟体液中孵育以制备稳定的涂层。
