Department of Cranio-Maxillofacial Surgery, Oral Biotechnology & Bioengineering, University Hospital Zurich, Zurich, Switzerland.
J Biomed Mater Res A. 2010 Sep 15;94(4):1023-33. doi: 10.1002/jbm.a.32773.
The potential use as a bone substitute material of a three-dimensional bioactive glass fiber scaffold composed of Na(2)O-K(2)O-MgO-CaO-B(2)O(3)-P(2)O(5)-SiO(2) (BG1) was investigated in this work. Scaffolds were pre-treated with simulated body fluid (SBF) to promote the formation of two different bone-like apatite layers on their surfaces. The topography and roughness of the deposited layers were assessed by scanning electron microscopy (SEM), while the chemical composition and structure using X-ray photoelectron spectroscopy (XPS) and Raman spectroscopy, respectively. Based on surface analysis, the bioactive glass surfaces were ranked from smoothest to roughest: 0 SBF (untreated), 1x SBF and 2x SBF. A calcium-deficient carbonated hydroxyapatite (HCA) layer was present on both SBF-treated scaffolds, with higher number and larger bone-like apatite nodule formation in the 2x SBF case. MC3T3-E1 preosteoblasts showed a more flattened morphology and higher cell proliferation on the nontreated scaffolds; whereas, cells were more elongated and had higher osteoblastic activity on SBF-treated samples. In vivo results in a rabbit calvarial bone defect model showed enhanced bone formation with SBF pretreated scaffolds, compared with untreated ones, commercially available Perioglass particles and empty defects. Our findings demonstrate that the formation of a rough HCA layer on bioactive glass porous scaffolds enhanced preosteoblast maturation in vitro, as well as bone formation in vivo.
本研究旨在探讨由 Na(2)O-K(2)O-MgO-CaO-B(2)O(3)-P(2)O(5)-SiO(2)（BG1）组成的三维生物活性玻璃纤维支架作为骨替代材料的潜力。支架先用模拟体液（SBF）进行预处理，以促进在其表面形成两种不同的类骨质磷灰石层。通过扫描电子显微镜（SEM）评估沉积层的形貌和粗糙度，通过 X 射线光电子能谱（XPS）和拉曼光谱分别评估化学成分和结构。基于表面分析，生物活性玻璃表面的粗糙度从低到高依次为：0 SBF（未处理）、1x SBF 和 2x SBF。两种 SBF 处理的支架上均存在缺钙碳酸羟基磷灰石（HCA）层，2x SBF 情况下形成了更多数量和更大的类骨质磷灰石结节。MC3T3-E1 前成骨细胞在未经处理的支架上表现出更扁平的形态和更高的细胞增殖；而在 SBF 处理的样品上，细胞更细长，成骨细胞活性更高。在兔颅骨骨缺损模型的体内结果表明，与未处理组相比，用 SBF 预处理的支架可增强骨形成，与商业可得的 Perioglass 颗粒和空缺陷相比也是如此。我们的研究结果表明，在生物活性玻璃多孔支架上形成粗糙的 HCA 层可增强体外前成骨细胞成熟，以及体内骨形成。
