Lin Yuan, Wang Luling, Zhang Peibiao, Wang Xin, Chen Xuesi, Jing Xiabin, Su Zhaohui
State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin 130022, China.
Acta Biomater. 2006 Mar;2(2):155-64. doi: 10.1016/j.actbio.2005.10.002. Epub 2005 Dec 7.
Poly(L-lactide) (PLLA) surface was modified via aminolysis by poly(allylamine hydrochloride) (PAH) at high pH and subsequent electrostatic self-assembly of poly(sodium styrenesulfonate) (PSS) and PAH, and the process was monitored by X-ray photoelectron spectroscopy (XPS) and contact angle measurement. These modified PLLAs were then used as charged substrates for further incorporation of gelatin to improve their cytocompatibility. The amphoteric nature of the gelatin was exploited and the gelatin was adsorbed to the negatively charged PLLA/PSS and positively charged PLLA/PAH at pH=3.4 and 7.4, respectively. XPS and water contact angle data indicated that the gelatin adsorption at pH=3.4 resulted in much higher surface coverage by gelatin than at pH=7.4. All the modified PLLA surfaces became more hydrophilic than the virgin PLLA. Chondrocyte culture was used to test the cell attachment, cell morphology and cell viability on the modified PLLA substrates. The results showed that the PAH and PSS modified PLLA exhibited better cytocompatibility than virgin PLLA, and the incorporation of the gelatin on these modified PLLA substrates further improved their cytocompatibility, with the PLLA/PSS substrate treated with the gelatin at pH=3.4 being the best, exceeding the chondrocyte compatibility of the tissue culture polystyrene.
聚（L-丙交酯）（PLLA）表面通过在高pH值下用聚（烯丙胺盐酸盐）（PAH）进行氨解，随后进行聚（苯乙烯磺酸钠）（PSS）和PAH的静电自组装来改性，该过程通过X射线光电子能谱（XPS）和接触角测量进行监测。然后将这些改性的PLLA用作带电荷的底物，进一步掺入明胶以改善其细胞相容性。利用明胶的两性性质，在pH = 3.4和7.4时，明胶分别吸附到带负电荷的PLLA / PSS和带正电荷的PLLA / PAH上。XPS和水接触角数据表明，在pH = 3.4时明胶的吸附导致明胶在表面上的覆盖度远高于pH = 7.4时的情况。所有改性的PLLA表面都比原始PLLA更亲水。使用软骨细胞培养来测试改性PLLA底物上的细胞附着、细胞形态和细胞活力。结果表明，PAH和PSS改性的PLLA比原始PLLA表现出更好的细胞相容性，并且在这些改性的PLLA底物上掺入明胶进一步改善了它们的细胞相容性，其中在pH = 3.4时用明胶处理的PLLA / PSS底物效果最佳，超过了组织培养聚苯乙烯的软骨细胞相容性。
