Ma Zuwei, Gao Changyou, Gong Yihong, Ji Jian, Shen Jiacong
Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
J Biomed Mater Res. 2002;63(6):838-47. doi: 10.1002/jbm.10470.
With the use of a grafting-coating method, three kinds of natural macromolecules, that is, gelatin, collagen, or chitosan, were immobilized on poly-L-lactic acid (PLLA) membrane surfaces with the goal of improving of cellular interactions. Attenuated total reflectance infrared spectroscopy (ATR-IR), x-ray photoelectron spectroscopy (XPS) and surface morphology analysis using scanning electronic microscopy (SEM) confirmed that the natural macromolecule layers adhered tightly to the hydrophobic PLLA membrane surfaces. Chondrocyte culture showed that the modified PLLA membranes had higher cell attachment, higher cell proliferation rate, and higher cell activity than the control PLLA membrane. Moreover, the chondrocytes were more spread out on the modified PLLA membranes than on the control PLLA membranes.
采用接枝涂层法，将三种天然大分子，即明胶、胶原蛋白或壳聚糖固定在聚-L-乳酸（PLLA）膜表面，目的是改善细胞相互作用。衰减全反射红外光谱（ATR-IR）、X射线光电子能谱（XPS）以及使用扫描电子显微镜（SEM）进行的表面形态分析证实，天然大分子层紧密附着在疏水性PLLA膜表面。软骨细胞培养表明，与对照PLLA膜相比，改性PLLA膜具有更高的细胞附着率、更高的细胞增殖率和更高的细胞活性。此外，软骨细胞在改性PLLA膜上比在对照PLLA膜上铺展得更开。
