Tort Adriano B L, Neto Waldemar P, Amaral Olavo B, Kazlauckas Vanessa, Souza Diogo O, Lara Diogo R
Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600, Prédio Anexo. Lab. 26, Porto Alegre, RS, Brazil.
J Neurosci Methods. 2006 Oct 15;157(1):91-7. doi: 10.1016/j.jneumeth.2006.04.005. Epub 2006 May 15.
We hereby describe a simple and inexpensive approach to evaluate the position and locomotion of rodents in an arena. The system is based on webcam registering of animal behaviour with subsequent analysis on customized software. Based on black/white differentiation, it provides rapid evaluation of animal position over a period of time, and can be used in a myriad of behavioural tasks in which locomotion, velocity or place preference are variables of interest. A brief review of the results obtained so far with this system and a discussion of other possible applications in behavioural neuroscience are also included. Such a system can be easily implemented in most laboratories and can significantly reduce the time and costs involved in behavioural analysis, especially in developing countries.
我们在此描述一种简单且成本低廉的方法,用于评估啮齿动物在实验场地中的位置和运动情况。该系统基于网络摄像头对动物行为的记录,并随后在定制软件上进行分析。基于黑白区分,它能在一段时间内快速评估动物的位置,并且可用于众多行为任务,其中运动、速度或位置偏好是感兴趣的变量。本文还简要回顾了使用该系统迄今所获得的结果,并讨论了其在行为神经科学中的其他可能应用。这样的系统在大多数实验室中都能轻松实现,并且可以显著减少行为分析所涉及的时间和成本,尤其是在发展中国家。