Kautiainen A, Törnqvist M, Anderstam B, Vaca C E
Department of Radiobiology, Arrhenius Laboratories for Natural Sciences, Stockholm University, Sweden.
Carcinogenesis. 1991 Jun;12(6):1097-102. doi: 10.1093/carcin/12.6.1097.
Hemoglobin (Hb) adduct determination by the N-alkyl Edman method was used for in vivo dosimetry of endogenously formed malonaldehyde (MA) and ethene in mice fed diets with different fatty acid composition and induced for lipid peroxidation with carbon tetrachloride (CCl4). In order to amplify lipid peroxidation animals were pretreated with phenobarbital (PB) and the glutathione-depleting agent DL-buthionine-(S,R)-sulfoximine (BSO). Non-treated animals raised on different diets were used as controls. Lipid peroxidation products in liver were measured as 2-thiobarbituric acid reactive compounds (TBA-C). Livers from control mice fed a soya oil based diet (rich in polyunsaturated fatty acids, diet S) showed approximately 6.5-fold higher levels of TBA-C than those from animals raised on a coconut oil based diet (mostly saturated fatty acids, diet C). The level of adducts of MA to Hb, determined as N-(3-hydroxypropyl)valine, was approximately 1.5-fold higher in animals from diet S than in animals raised on diet C. The highest increases in the levels of TBA-C and MA adducts were obtained after a simultaneous treatment of the animals with PB, BSO and CCl4. The increases of TBA-C were 1.3-fold (diet C) and 1.7-fold (diet S). The corresponding increases of MA-Hb adduct levels were 1.3- and 1.6-fold respectively, indicating an increased susceptibility of mice fed diet S to lipid peroxidation. The level of adducts from ethene, determined as N-(2-hydroxyethyl)valine, was also higher in mice from diet S than in animals fed diet C, when all treatment groups were considered. The difference was, however, only slightly significant (P less than 0.02). No difference between control and CCl4-treated animals, with regard to the ethene-Hb adduct, was found. Our results validate the use of Hb dosimetry for monitoring the effects of factors known to influence lipid peroxidation induced in vivo.
采用N - 烷基埃德曼法测定血红蛋白（Hb）加合物，用于对喂食不同脂肪酸组成日粮并经四氯化碳（CCl4）诱导脂质过氧化的小鼠体内内源性生成的丙二醛（MA）和乙烯进行体内剂量测定。为了增强脂质过氧化作用，对动物进行苯巴比妥（PB）和谷胱甘肽消耗剂DL - 丁硫氨酸 - （S，R） - 亚砜亚胺（BSO）预处理。以不同日粮饲养的未处理动物用作对照。肝脏中的脂质过氧化产物以2 - 硫代巴比妥酸反应性化合物（TBA - C）进行测定。喂食大豆油基日粮（富含多不饱和脂肪酸，日粮S）的对照小鼠肝脏中TBA - C水平比喂食椰子油基日粮（主要是饱和脂肪酸，日粮C）的动物肝脏中的TBA - C水平高约6.5倍。以N - （3 - 羟丙基）缬氨酸测定的MA与Hb加合物水平，日粮S组动物比日粮C组动物高约1.5倍。在用PB、BSO和CCl4同时处理动物后，TBA - C和MA加合物水平升高幅度最大。TBA - C的升高幅度分别为1.3倍（日粮C）和1.7倍（日粮S）。MA - Hb加合物水平相应升高分别为1.3倍和1.6倍，表明喂食日粮S的小鼠对脂质过氧化更敏感。当考虑所有处理组时，以N - （2 - 羟乙基）缬氨酸测定的乙烯加合物水平在日粮S组小鼠中也高于日粮C组小鼠。然而，差异仅略有显著性（P小于0.02）。未发现对照动物和CCl4处理动物在乙烯 - Hb加合物方面存在差异。我们的结果验证了Hb剂量测定法可用于监测已知影响体内诱导脂质过氧化的因素的作用。
