Pleshkewych A, Levine L
J Cell Sci. 1975 Jun;18(1):1-17. doi: 10.1242/jcs.18.1.1.
A prominent cytoplasmic inclusion present in living mouse primary spermatocytes has been observed by both light and electron microscopy. It began to form at prometaphase and continued to increase in thickness and length as the cells developed. By metaphase it was a distinct sausage-shaped boundary that enclosed a portion of the cytoplasm between the spindle and the cell membrane. At the end of metaphase, the inclusion reached its maximum length. At telophase, it was divided between the daughter secondaries. The inclusion persisted as a circular contour in the interphase secondary spermatocyte. Electron microscopy of the same cultured cells that were previously observed with light microscopy revealed that the inclusion was a distinctive formation of membranes. It consisted of agranular cisternae and vesicles, and was therefore a membranous complex. Many of the smaller vesicles in the membranous complex resembled those found in the spindle. The cisternae in the membranous complex were identical to the cisternal endoplasmic reticulum of interphase primary spermatocytes. Nevertheless, the organization of vesicles and cisternae into the membranous complex was unique for the primaries in division stages, since such an organization was not present in their interphase stages.
通过光学显微镜和电子显微镜观察到，活的小鼠初级精母细胞中存在一种显著的细胞质内含物。它在有丝分裂前期开始形成，并随着细胞发育，其厚度和长度持续增加。到中期时，它成为一个明显的香肠状边界，包围着纺锤体和细胞膜之间的一部分细胞质。在中期结束时，内含物达到其最大长度。在末期，它被分配到子二级精母细胞中。内含物在间期二级精母细胞中以圆形轮廓持续存在。对先前用光学显微镜观察过的相同培养细胞进行电子显微镜观察发现，内含物是一种独特的膜结构。它由无颗粒的扁平囊和小泡组成，因此是一种膜性复合体。膜性复合体中的许多较小的小泡类似于纺锤体中的小泡。膜性复合体中的扁平囊与间期初级精母细胞的扁平囊内质网相同。然而，小泡和扁平囊组织成膜性复合体对于分裂期的初级精母细胞来说是独特的，因为在它们的间期阶段不存在这样的组织。
