Kulkarni Savita, Vyas Soumil, Supe Avinash, Kadival Gururaj
Laboratory Nuclear Medicine Section, Isotope Group, Bhabha Atomic Research Center, C/o T.M.H. Annexe, Mumbai, India.
J Gastroenterol Hepatol. 2006 May;21(5):819-23. doi: 10.1111/j.1440-1746.2006.04030.x.
Early diagnosis and prompt treatment of abdominal tuberculosis is vitally important as it greatly reduces disease and treatment related morbidity and even mortality in extreme cases. A polymerase chain reaction (PCR) test was evaluated for its feasibility as a diagnostic tool in abdominal tuberculosis (TB) in the Indian scenario.
PCR for the identification of M. tuberculosis amplified a 340 bp nucleotide sequence located within the 38 kDa protein gene of M. tuberculosis. Tissues for processing were obtained from patients suspected to have abdominal TB. These were from various sources such as abdominal lymph nodes, segments of intestine and bowel obtained at various times and in different ways such as laparoscopy, colectomy, bowel and lymph node resection. Fifty such patients had their tissues sent for PCR.
PCR results were compared with histopathology (HP). Of the 50 samples, 31 were positive for abdominal TB by HP whereas 30 were positive by PCR. Twenty-four of these were positive for both HP and PCR while of the seven samples positive for HP, five were negative and two gave inhibition by PCR. Six samples negative by HP were positive by PCR.
This study demonstrates that PCR can be used as an effective tool to diagnose abdominal TB.
腹部结核的早期诊断和及时治疗至关重要，因为这能极大降低疾病及与治疗相关的发病率，在极端情况下甚至可降低死亡率。在印度的情况下，对聚合酶链反应（PCR）检测作为腹部结核诊断工具的可行性进行了评估。
用于鉴定结核分枝杆菌的PCR扩增了位于结核分枝杆菌38 kDa蛋白基因内的一段340 bp核苷酸序列。用于检测的组织取自疑似患有腹部结核的患者。这些组织来源多样，如腹部淋巴结、不同时间以不同方式获取的肠段，包括通过腹腔镜检查、结肠切除术、肠和淋巴结切除术获取的组织。50例此类患者的组织被送去进行PCR检测。
将PCR结果与组织病理学（HP）结果进行比较。在50个样本中，HP检测显示31个样本腹部结核呈阳性，而PCR检测显示30个样本呈阳性。其中24个样本HP和PCR检测均为阳性，在HP检测呈阳性的7个样本中，5个样本PCR检测为阴性，2个样本PCR检测出现抑制情况。HP检测呈阴性的6个样本PCR检测为阳性。
本研究表明PCR可作为诊断腹部结核的有效工具。
