用于快速诊断胃肠道结核的多重聚合酶链反应
Multiplex PCR for rapid diagnosis of gastrointestinal tuberculosis.
作者信息
Sharma Kusum, Sinha Saroj Kant, Sharma Aman, Nada Ritambra, Prasad Kaushal K, Goyal Kapil, Rana Surinder Singh, Bhasin Deepak Kumar, Sharma Meera
机构信息
Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
出版信息
J Glob Infect Dis. 2013 Apr;5(2):49-53. doi: 10.4103/0974-777X.112272.
BACKGROUND
Rapid and specific diagnosis of gastrointestinal tuberculosis (GITB) is of utmost importance.
AIM
To evaluate Multiplex PCR (MPCR) using MPB64 and IS6110 primers specific for M. tuberculosis for rapid diagnosis of GITB.
MATERIALS AND METHODS
MPCR was performed on colonoscopy biopsy specimens on 11 GITB confirmed (culture/AFB/histopathology was positive), 29 GITB suspected and 30 Non GITB (control group) patients.
RESULTS
MPB64 PCR had sensitivity and specificity of 90% and 100% for confirmed GITB cases. In 29 clinically diagnosed but unconfirmed GITB cases, MPCR was positive in 72.41%. MPCR was negative in all control group patients. The overall sensitivity and specificity of microscopy, culture, histopathology and MPCR was 5%, 2% 20% and 77.5% and 100%, 100%, 100% and 100% respectively.
CONCLUSION
MPCR has good sensitivity and specificity in diagnosing gastrointestinal tuberculosis.
背景
胃肠道结核（GITB）的快速和特异性诊断至关重要。
目的
评估使用针对结核分枝杆菌的MPB64和IS6110引物的多重聚合酶链反应（MPCR）对胃肠道结核进行快速诊断。
材料与方法
对11例确诊的GITB（培养/抗酸杆菌/组织病理学呈阳性）、29例疑似GITB患者以及30例非GITB患者（对照组）的结肠镜活检标本进行MPCR检测。
结果
对于确诊的GITB病例，MPB64 PCR的敏感性和特异性分别为90%和100%。在29例临床诊断但未确诊的GITB病例中，MPCR阳性率为72.41%。所有对照组患者的MPCR均为阴性。显微镜检查、培养、组织病理学和MPCR的总体敏感性和特异性分别为5%、2%、20%和77.5%，以及100%、100%、100%和100%。
结论
MPCR在诊断胃肠道结核方面具有良好的敏感性和特异性。
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