Sánchez Mendoza M, Fernández Rendón E, Mota de la Garza L
Departamento de Microbiología de la Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México, D.F., México.
Rev Latinoam Microbiol. 1991 Apr-Sep;33(2-3):145-8.
There are many media recommended for the isolation of Staphylococcus aureus from foods, but only with some media one can obtain a good growth started with stressed cells. The Baird Parker (BP) medium is considered the best choice to recover stressed cells, however, it is not as good a medium to isolate Staphylococcus aureus from powder milk. Therefore, it is important to count with alternative media to enhance the chance for Staphylococcus aureus to grow from dehydrated products. Thirty-one powder milk samples contaminated with Staphylococcus aureus were analysed by Baird Parker method, employing four culture media: Baird Parker (BP), Baird Parker + tween + MgCl2 (BPTM), Pork plasma with bovine fibrinogen agar (PPF) and Salt Milk agar (SL). Staphylococcus aureus was isolated in SL, 38.7%; in BP, 3.2%; in BPTM, 6.4%; and PPF, 0%.
有许多用于从食品中分离金黄色葡萄球菌的培养基,但只有部分培养基能使应激细胞良好生长。贝尔德·帕克(BP)培养基被认为是复苏应激细胞的最佳选择,然而,它并非从奶粉中分离金黄色葡萄球菌的理想培养基。因此,拥有替代培养基以增加从脱水产品中培养出金黄色葡萄球菌的机会很重要。采用四种培养基:贝尔德·帕克(BP)、贝尔德·帕克+吐温+氯化镁(BPTM)、含牛纤维蛋白原的猪血浆琼脂(PPF)和盐奶琼脂(SL),通过贝尔德·帕克方法对31份被金黄色葡萄球菌污染的奶粉样品进行分析。在SL中分离出金黄色葡萄球菌的比例为38.7%;在BP中为3.2%;在BPTM中为6.4%;在PPF中为0%。