使用改良的贝尔德-帕克培养基检测大容量冷藏罐牛奶中的金黄色葡萄球菌。
Detection of Staphylococcus aureus in bulk tank milk using modified Baird-Parker culture media.
作者信息
Ollis G W, Rawluk S A, Schoonderwoerd M, Schipper C
机构信息
Animal Industry Division, Alberta Agriculture, Food and Rural Development, Edmonton.
出版信息
Can Vet J. 1995 Oct;36(10):619-23.
The purpose of this project was to evaluate the use of 2 selective/differential culture media for detecting Staphylococcus aureus in bulk tank milk. One medium was Baird-Parker agar base supplemented with egg york tellurite emulsion and acriflavine. The other medium was Baird-Parker agar base supplemented with rabbit plasma/bovine fibrinogen and acriflavine. An increased inoculum of bulk tank milk (0.3 mL) was used to enhance the detection of S. aureus in samples containing low numbers of organisms. The sensitivity and specificity for detecting S. aureus in bulk tank milk were 94.8% and 100%, respectively, using Baird-Parker agar base supplemented with egg yolk tellurite emulsion and acriflavine, and 89.7% and 100%, respectively, using Baird-Parker agar base supplemented with rabbit plasma/bovine fibrinogen and acriflavine. Both media are practical for detecting S. aureus in bulk tank milk and monitoring its spread in lactating dairy herds in Alberta.
本项目的目的是评估两种选择性/鉴别培养基用于检测大罐牛奶中金黄色葡萄球菌的效果。一种培养基是添加了蛋黄亚碲酸盐乳剂和吖啶黄的贝尔德-帕克琼脂基础培养基。另一种培养基是添加了兔血浆/牛纤维蛋白原和吖啶黄的贝尔德-帕克琼脂基础培养基。使用增加接种量的大罐牛奶（0.3 mL）来提高在含菌量低的样品中检测金黄色葡萄球菌的能力。使用添加了蛋黄亚碲酸盐乳剂和吖啶黄的贝尔德-帕克琼脂基础培养基检测大罐牛奶中金黄色葡萄球菌的灵敏度和特异性分别为94.8%和100%，而使用添加了兔血浆/牛纤维蛋白原和吖啶黄的贝尔德-帕克琼脂基础培养基时，灵敏度和特异性分别为89.7%和100%。这两种培养基对于检测艾伯塔省泌乳奶牛群大罐牛奶中的金黄色葡萄球菌及其传播监测均具有实用性。
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