Uscanga-Prieto I, Fernández-Rendón E, Mota de la Garza L
Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México, D.F., Mexico.
Rev Latinoam Microbiol. 1994 Jul-Sep;36(3):191-6.
Fifty-one cooked ham samples were analized by three methods for investigation of Staphylococcus aureus; Modified Van Doorne and American Public Health Association; The Official Mexican Method. The first two are enrichment methods and in all three a comparison between Baird Parker agar and salt milk agar was done. The modified Van Doorne technique was the best for isolation of S. aureus from cooked ham. In Baird Parker agar it was possible to demonstrate the presence of S. aureus in all positive samples. The study shows the importance of using Baird Parker broth as an enrichment medium for the isolation of S. aureus from products in which a thermal treatment and addition of salts as sodium chloride and nitrites, inhibit the growth of this microorganism.
采用三种方法对51份熟火腿样本进行了金黄色葡萄球菌检测,分别是改良范多恩法、美国公共卫生协会法和墨西哥官方方法。前两种是增菌法,并且在所有三种方法中都比较了贝尔德·帕克琼脂和盐乳琼脂。改良范多恩技术是从熟火腿中分离金黄色葡萄球菌的最佳方法。在贝尔德·帕克琼脂上,所有阳性样本都能检测出金黄色葡萄球菌的存在。该研究表明,对于从经过热处理以及添加了氯化钠和亚硝酸盐等盐类从而抑制该微生物生长的产品中分离金黄色葡萄球菌而言,使用贝尔德·帕克肉汤作为增菌培养基非常重要。