Matveev I V, Shaposhnikova T G, Podgornaya O I
Institute of Cytology RAS, Tikhoretsky pr. 4, St-Petersburg 194064, Russia.
Gene. 2007 Sep 1;399(1):20-5. doi: 10.1016/j.gene.2007.04.034. Epub 2007 May 10.
Body of the scyphoid jellyfish Aurelia aurita consists of 2 epithelia -- epidermis and gastroderm. The layers are separated by a thick layer of extracellular matrix -- mesoglea. A. aurita has a lot of cells in the mesoglea unlike many other Cnidarians. The major protein of the mesoglea with apparent molecular mass of 47 kDa was detected by SDS-PAGE. A partial mRNA of the protein 1421 bp long was cloned and sequenced. The search for homologous nucleotide and protein sequences shows that the mRNA sequence is novel. Deduced amino acid sequence of 416 aa contains zona pellucida (ZP) domain and Delta/Serrate/Lag-2 (DSL) domain. The protein was named mesoglein. According to reverse transcription PCR analysis it is expressed in the mature medusa exclusively in the mesogleal cells. Mesoglein belongs to the lowest phyla among ZP domain-containing proteins. The protein is supposed to be a structural element of the mesoglea extracellular matrix.
钵水母纲海月水母（Aurelia aurita）的身体由两层上皮组织构成——表皮和胃皮层。这两层组织被一层厚厚的细胞外基质——中胶层分隔开来。与许多其他刺胞动物不同，海月水母的中胶层中有大量细胞。通过SDS-PAGE检测到中胶层的主要蛋白质，其表观分子量为47 kDa。克隆并测序了该蛋白质1421 bp长的部分mRNA。对同源核苷酸和蛋白质序列的搜索表明，该mRNA序列是新的。推导的416个氨基酸的氨基酸序列包含透明带（ZP）结构域和Delta/Serrate/Lag-2（DSL）结构域。该蛋白质被命名为中胶蛋白。根据逆转录PCR分析，它仅在成熟水母的中胶层细胞中表达。中胶蛋白属于含ZP结构域蛋白质中最低等的门类。该蛋白质被认为是中胶层细胞外基质的一种结构成分。
