Sadovskaya Irina, Chaignon Philippe, Kogan Grigorij, Chokr Ali, Vinogradov Evgeny, Jabbouri Saïd
Laboratoire de Recherche sur les Biomatériaux et les Biotechnologies INSERM-ERI 002, Université du Littoral-Côte d'Opale, Boulogne-sur-mer, France.
FEMS Immunol Med Microbiol. 2006 Jun;47(1):75-82. doi: 10.1111/j.1574-695X.2006.00068.x.
The capacity of coagulase-negative staphylococci to colonize implanted medical devices is generally attributed to their ability to produce biofilms. Biofilm of the model strain of Staphylococcus epidermidis RP62A was shown to contain two carbohydrate-containing moieties, a linear poly-beta-(1-->6)-N-acetyl-D-glucosamine (PNAG) and teichoic acid. In the present study, we investigated several biofilm-producing staphylococci isolated from infected orthopaedic implants and characterized the composition of the laboratory-grown biofilms using chemical analysis and 1H nuclear magnetic resonance spectroscopy. Extracellular teichoic acid was produced by all strains studied. Some of the clinical strains were shown to produce biofilms with compositions similar to that of the model strain, containing a varying amount of PNAG. The chemical structure of PNAG of the clinical strains was similar to that previously described for the model strains S. epidermidis RP62A and Staphylococcus aureus MN8m, differing only in the amount of charged groups. Biofilms of the strains producing a substantial amount of PNAG were detached by dispersin B, a PNAG-degrading enzyme, while being unsusceptible to proteinase K treatment. On the other hand, some strains produced biofilms without any detectable amount of PNAG. The biofilms of these strains were dispersed by proteinase K, but not by dispersin B.
凝固酶阴性葡萄球菌在植入式医疗器械上定殖的能力通常归因于其产生生物膜的能力。表皮葡萄球菌RP62A模型菌株的生物膜显示含有两个含碳水化合物的部分，一种线性的聚-β-(1→6)-N-乙酰-D-葡萄糖胺（PNAG）和磷壁酸。在本研究中，我们调查了从感染的骨科植入物中分离出的几种产生生物膜的葡萄球菌，并使用化学分析和1H核磁共振光谱对实验室培养的生物膜的组成进行了表征。所有研究的菌株都产生细胞外磷壁酸。一些临床菌株被证明产生的生物膜组成与模型菌株相似，含有不同量的PNAG。临床菌株PNAG的化学结构与先前描述的模型菌株表皮葡萄球菌RP62A和金黄色葡萄球菌MN8m相似，只是带电基团的数量不同。产生大量PNAG的菌株的生物膜可被PNAG降解酶分散素B分离，而对蛋白酶K处理不敏感。另一方面，一些菌株产生的生物膜中没有可检测到的PNAG。这些菌株的生物膜可被蛋白酶K分散，但不能被分散素B分散。
