Kongo J M, Gomes A M, Malcata F X
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, Porto, Portugal.
Lett Appl Microbiol. 2006 Jun;42(6):595-9. doi: 10.1111/j.1472-765X.2006.01882.x.
This work was undertaken to study the feasibility and the characteristics of a fermented product made of goat milk, using a mixed starter culture of Bifidobacterium animalis and Lactobacillus acidophilus under controlled conditions, and to determine their survival in the fermented milk during refrigerated storage.
Goat milk was inoculated with Lact. acidophilus and Bif. animalis mixed starter, fermented in a glass bioreactor with controlled temperature (37 degrees C) and anaerobiosis, and monitored for growth and acidification. The fermented milk was then stored for 10 days under refrigeration, and monitored daily for starter microflora survival and pH changes. Lact. acidophilus viable counts reached a maximum of 7.1 x 10(8) colony-forming units (CFU) ml(-1), and Bif. animalis a maximum of 6.3 x 10(7) CFU ml(-1) by 20 h of fermentation. During refrigerated storage, both strains exhibited a good survival, with viable numbers remaining essentially constant throughout the experiment, whereas the pH of the fermented milk dropped slightly.
Mixed cultures of Bif. animalis and Lact. acidophilus may be used to produce fermented goat milk with high counts of both probiotic strains.
Goat milk fermented with Bif. animalis and Lact. acidophilus can be manufactured as an alternative probiotic dairy product.
本研究旨在探讨在可控条件下,使用动物双歧杆菌和嗜酸乳杆菌的混合发酵剂培养物制作山羊奶发酵产品的可行性和特性,并确定它们在冷藏储存期间在发酵乳中的存活情况。
将嗜酸乳杆菌和动物双歧杆菌混合发酵剂接种到山羊奶中,在温度可控(37摄氏度)且厌氧的玻璃生物反应器中发酵,并监测其生长和酸化情况。然后将发酵乳冷藏储存10天,每天监测发酵剂微生物的存活情况和pH值变化。发酵20小时时,嗜酸乳杆菌的活菌数最高达到7.1×10⁸ 菌落形成单位(CFU)/毫升,动物双歧杆菌最高达到6.3×10⁷ CFU/毫升。在冷藏储存期间,两种菌株均表现出良好的存活率,在整个实验过程中活菌数基本保持恒定,而发酵乳的pH值略有下降。
动物双歧杆菌和嗜酸乳杆菌的混合培养物可用于生产含有高数量两种益生菌菌株的发酵山羊奶。
用动物双歧杆菌和嗜酸乳杆菌发酵的山羊奶可作为一种替代性的益生菌乳制品进行生产。