Li Yonghong, Wang Yun, Liu Yingying, Li Xuan, Feng Lifei, Li Keke
Key Laboratory of Advanced Drug Preparation Technologies, Ministry of Education, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, 450001, Henan, China.
Henan Province Collaborative Innovation Center of New Drug Research and Safety Evaluation, Zhengzhou, 456150, Henan, China.
AMB Express. 2022 Feb 15;12(1):19. doi: 10.1186/s13568-022-01354-5.
Clostridium butyricum is a spore-forming probiotic which can promote the enhancement of beneficial bacteria and maintain intestinal microecological balance. However, it is difficult to improve the production level of C. butyricum by conventional fermentation process. In this study, a co-fermentation process of C. butyricum DL-1 and Bacillus coagulans ZC2-1 was established to improve the viable counts and spore yield of C. butyricum, and the formula of coculture medium was optimized by flask fermentation. The results showed that the optimum medium composition is 10 g/L bran, 15 g/L corn steep powder, 15 g/L peptone, 1 g/L KHPO and 0.5 g/L MnSO.Cultured stationarily in the optimal medium for 36 h, the number of viable bacteria of C. butyricum DL-1 reached 1.5 × 10 cfu/mL, Which was 375 times higher than that incubated in the initial medium. The sporulation rate reach 92.6%. The results revealed an economical and effective medium composition for the coculture of C. butyricum and B. coagulans, which achieved a 64.6% cost reduction. The co-fermentation process established in this study provides a new fermentation mode for the industrial production of other absolute anerobic bacteria.
丁酸梭菌是一种形成芽孢的益生菌，可促进有益菌的增加并维持肠道微生态平衡。然而，通过传统发酵工艺提高丁酸梭菌的生产水平较为困难。在本研究中，建立了丁酸梭菌DL-1和凝结芽孢杆菌ZC2-1的共发酵工艺，以提高丁酸梭菌的活菌数和芽孢产量，并通过摇瓶发酵优化了共培养基配方。结果表明，最佳培养基组成为10 g/L麸皮、15 g/L玉米浆干粉、15 g/L蛋白胨、1 g/L磷酸氢二钾和0.5 g/L硫酸锰。在最佳培养基中静置培养36 h，丁酸梭菌DL-1的活菌数达到1.5×10⁹ cfu/mL，比在初始培养基中培养的活菌数高375倍。芽孢形成率达到92.6%。结果揭示了一种用于丁酸梭菌和凝结芽孢杆菌共培养的经济有效的培养基组成，成本降低了64.6%。本研究建立的共发酵工艺为其他严格厌氧菌的工业化生产提供了一种新的发酵模式。
