Waragai Masaaki, Wei Jianshe, Fujita Masayo, Nakai Masaaki, Ho Gilbert J, Masliah Eliezer, Akatsu Hiroyasu, Yamada Tatsuo, Hashimoto Makoto
Laboratory for Chemistry and Metabolism, Tokyo Metropolitan Institute for Neuroscience, Tokyo 183-8526, Japan.
Biochem Biophys Res Commun. 2006 Jul 7;345(3):967-72. doi: 10.1016/j.bbrc.2006.05.011. Epub 2006 May 11.
DJ-1 is an antioxidant protein whose loss of function by gene mutations has been linked to familial Parkinson's disease (PD). The main objective of the present study was to determine if this molecule was also involved in the pathogenesis of sporadic PD. For this purpose, quantitative immunoblot assays were performed to evaluate DJ-1 in cerebrospinal fluids (CSF) collected from sporadic PD patients (n=40) and non-PD controls (n=38). The results showed that the CSF DJ-1 levels in PD were significantly higher than those in non-PD controls. Especially, upregulation of CSF DJ-1 in the early stage of PD (Yahr I-II) were distinct compared to those in the advanced stage of PD (Yahr III-IV) and non-PD controls (p<0.001 by ANOVA with post hoc Bonferroni's test), suggesting a protective role of DJ-1 against oxidative stress during the early stage. Thus, we propose that CSF DJ-1 could be a possible biomarker for early sporadic PD.
DJ-1是一种抗氧化蛋白，其因基因突变导致的功能丧失与家族性帕金森病（PD）有关。本研究的主要目的是确定该分子是否也参与散发性PD的发病机制。为此，进行了定量免疫印迹分析，以评估从散发性PD患者（n = 40）和非PD对照（n = 38）收集的脑脊液（CSF）中的DJ-1。结果显示，PD患者脑脊液中DJ-1水平显著高于非PD对照。特别是，与PD晚期（Yahr III-IV）和非PD对照相比，PD早期（Yahr I-II）脑脊液中DJ-1的上调明显（方差分析后经Bonferroni检验，p<0.001），表明DJ-1在早期对氧化应激具有保护作用。因此，我们认为脑脊液DJ-1可能是早期散发性PD的一个潜在生物标志物。
