Banik R M, Santhiagu A, Upadhyay S N
School of Biochemical Engineering, Institute of Technology, Banaras Hindu University, Varanasi 221 005, India.
Bioresour Technol. 2007 Mar;98(4):792-7. doi: 10.1016/j.biortech.2006.03.012. Epub 2006 May 16.
A molasses based medium for the production of gellan by Sphingomonas paucimobilis ATCC-31461 was developed. Placket-Burman design criterion was applied to study the effect of various nutrient supplements on gellan production using molasses. Among the 20 variables tested, molasses, tryptone, casaminoacid, disodium hydrogen orthophosphate and manganese chloride showed significant effect on gellan production. A central composite design was applied to determine the optimum concentrations of the significant variables obtained from Placket-Burman design. Most suitable medium composition for production of gellan was (g/l): molasses-112.5; tryptone-1; casaminoacid-1; disodium hydrogen orthophosphate-1; manganese chloride-0.947 and the optimum gellan production was 13.814 g/l.
开发了一种用于少动鞘氨醇单胞菌ATCC - 31461生产结冷胶的基于糖蜜的培养基。采用Placket - Burman设计准则研究了各种营养补充剂对利用糖蜜生产结冷胶的影响。在测试的20个变量中，糖蜜、胰蛋白胨、酪蛋白氨基酸、磷酸氢二钠和氯化锰对结冷胶生产有显著影响。应用中心复合设计来确定从Placket - Burman设计中获得的显著变量的最佳浓度。生产结冷胶最合适的培养基组成（g / l）为：糖蜜 - 112.5；胰蛋白胨 - 1；酪蛋白氨基酸 - 1；磷酸氢二钠 - 1；氯化锰 - 0.947，最佳结冷胶产量为13.814 g / l。
