鼠伤寒沙门氏菌中黄苷的利用可通过黄苷磷酸化酶中单个天冬氨酸到甘氨酸的替换得以恢复。
Xanthosine utilization in Salmonella enterica serovar Typhimurium is recovered by a single aspartate-to-glycine substitution in xanthosine phosphorylase.
作者信息
Hansen Michael Riis, Tranekjaer Jørgensen Jesper, Dandanell Gert
机构信息
Department of Biological Chemistry, Institute of Molecular Biology and Physiology, University of Copenhagen, Sølvgade 83 H, 1307 Copenhagen K, Denmark.
出版信息
J Bacteriol. 2006 Jun;188(11):4153-7. doi: 10.1128/JB.01926-05.
Abstract
xapABR from Salmonella enterica was analyzed and compared with the corresponding Escherichia coli genes. xapB and xapR, but not xapA, encode functional proteins. An S. enterica XapA(Asp72Gly) mutant that restores the phosphorolytic activity was selected. The purified mutant enzyme has different kinetic constants than the E. coli enzyme but similar substrate specificity.
摘要
对来自肠炎沙门氏菌的xapABR进行了分析，并与相应的大肠杆菌基因进行了比较。xapB和xapR编码功能蛋白，而xapA不编码。筛选出了一种恢复磷酸解活性的肠炎沙门氏菌XapA(Asp72Gly)突变体。纯化后的突变酶与大肠杆菌酶具有不同的动力学常数，但底物特异性相似。
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本文引用的文献
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Escherichia coli purine nucleoside phosphorylase II, the product of the xapA gene.大肠杆菌嘌呤核苷磷酸化酶II，即xapA基因的产物。
J Mol Biol. 2005 Apr 22;348(1):113-25. doi: 10.1016/j.jmb.2005.02.019.
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Purification and characterization of RihC, a xanthosine-inosine-uridine-adenosine-preferring hydrolase from Salmonella enterica serovar Typhimurium.鼠伤寒沙门氏菌中一种优先作用于黄苷、肌苷、尿苷和腺苷的水解酶RihC的纯化与特性分析
Biochim Biophys Acta. 2005 May 25;1723(1-3):55-62. doi: 10.1016/j.bbagen.2005.01.012. Epub 2005 Feb 23.
3
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