大肠杆菌中参与黄苷分解代谢的基因（xapA、xapB和xapR）的鉴定与表征
Identification and characterization of genes (xapA, xapB, and xapR) involved in xanthosine catabolism in Escherichia coli.
作者信息
Seeger C, Poulsen C, Dandanell G
机构信息
Department of Biological Chemistry, University of Copenhagen, Denmark.
出版信息
J Bacteriol. 1995 Oct;177(19):5506-16. doi: 10.1128/jb.177.19.5506-5516.1995.
Abstract
We have characterized four genes from the 52-min region on the Escherichia coli linkage map. Three of these genes are directly involved in the metabolism of xanthosine, whereas the function of the fourth gene is unknown. One of the genes (xapA) encodes xanthosine phosphorylase. The second gene, named xapB, encodes a polypeptide that shows strong similarity to the nucleoside transport protein NupG. The genes xapA and xapB are located clockwise of a gene identified as xapR, which encodes a positive regulator belonging to the LysR family and is required for the expression of xapA and xapB. The genes xapA and xapB form an operon, and their expression was strictly dependent on the presence of both the XapR protein and the inducer xanthosine. Expression of the xapR gene is constitutive and not autoregulated, unlike the case for many other LysR family proteins. In minicells, the XapB polypeptide was found primarily in the membrane fraction, indicating that XapB is a transport protein like NupG and is involved in the transport of xanthosine.
摘要
我们已经对大肠杆菌连锁图谱上52分钟区域的四个基因进行了表征。其中三个基因直接参与黄苷的代谢，而第四个基因的功能尚不清楚。其中一个基因（xapA）编码黄苷磷酸化酶。第二个基因名为xapB，编码一种与核苷转运蛋白NupG有很强相似性的多肽。基因xapA和xapB位于一个名为xapR的基因的顺时针方向，xapR编码一种属于LysR家族的正调控因子，是xapA和xapB表达所必需的。基因xapA和xapB形成一个操纵子，它们的表达严格依赖于XapR蛋白和诱导物黄苷的同时存在。与许多其他LysR家族蛋白不同，xapR基因的表达是组成型的，不受自身调控。在小细胞中，发现XapB多肽主要存在于膜组分中，这表明XapB是一种像NupG一样的转运蛋白，参与黄苷的转运。
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