Wetton S, Hughes J, West N, Addy M
Division of Restorative Dentistry, University of Bristol, Dental School, Bristol, UK.
Caries Res. 2006;40(3):213-7. doi: 10.1159/000092228.
Previous research, mainly on enamel, supports a protective role for salivary pellicle against erosion. Pretreatments have tended to be lengthy (24 h or more) and of questionable relevance to the regular intake of acidic food and drink by many individuals. The aim of this study in vitro was to determine the protective effect of salivary pellicle formed on enamel and dentine over time periods up to 4 h. Flattened, polished human enamel and dentine specimens were pretreated with unstimulated human saliva from a single donor for 2 min, 30 min (enamel only), 1, 2, or 4 h. Controls were exposed to water for the same times. Specimens were then exposed to 0.3% citric acid, pH 3.2 for 10 min with stirring. This cycle was carried out 12 times. Tissue loss was measured by profilometry after 3, 6, 9 and 12 cycles. For enamel, statistically significant protection was found at >or=1 h. For dentine, significant protection was achieved at 2 min. Salivary pellicle offered proportionately greater protection to enamel than dentine. Cautiously extrapolating these in vitro data suggests that pellicle should offer erosion protection to individuals who imbibe acidic drinks at frequencies of 1 h or less.
以往主要针对牙釉质的研究支持唾液薄膜对侵蚀具有保护作用。预处理往往耗时较长（24小时或更长时间），而且对于许多人经常摄入酸性食物和饮料的情况而言，其相关性存疑。本体外研究的目的是确定在长达4小时的时间段内，在牙釉质和牙本质上形成的唾液薄膜的保护作用。将扁平、抛光的人牙釉质和牙本质标本用来自单一供体的未刺激人唾液预处理2分钟、30分钟（仅针对牙釉质）、1小时、2小时或4小时。对照组在相同时间内接触水。然后将标本在搅拌下暴露于pH值为3.2的0.3%柠檬酸中10分钟。此循环进行12次。在3次、6次、9次和12次循环后，通过轮廓测定法测量组织损失。对于牙釉质，在≥1小时时发现有统计学意义的保护作用。对于牙本质，在2分钟时即实现了显著的保护作用。唾液薄膜对牙釉质的保护作用比对牙本质的保护作用更大。谨慎推断这些体外数据表明，对于饮用酸性饮料频率为1小时或更低的个体，薄膜应能提供防侵蚀保护。
