Damian Lia-Raluca, Dumitrescu Ramona, Alexa Vlad Tiberiu, Focht David, Schwartz Cristoph, Balean Octavia, Jumanca Daniela, Obistioiu Diana, Lalescu Dacian, Stefaniga Sebastian-Aurelian, Berbecea Adina, Fratila Aurora Doris, Scurtu Alexandra Denisa, Galuscan Atena
Faculty of Dentistry, Department I, University of Medicine and Pharmacy "Victor Babes", Eftimie Murgu Sq. No. 2, 300041 Timisoara, Romania.
Translational and Experimental Clinical Research Centre in Oral Health, Department of Preventive, Community Dentistry and Oral Health, Faculty of Dentistry, University of Medicine and Pharmacy "Victor Babes", Eftimie Murgu Sq. No. 2, 300041 Timisoara, Romania.
Materials (Basel). 2022 Oct 17;15(20):7258. doi: 10.3390/ma15207258.
The aim of this study is to evaluate salivary remineralisation versus chemical remineralisation/infiltration of enamel, using different dentistry materials. The enamel changes were studied using confocal laser scanning microscopy (CLSM), and the depth of lesions and demineralisation/remineralisation/infiltration percentage were calculated. Additionally, the macro elemental composition of the teeth was performed using atomic absorption spectroscopy (AAS). Two studies were performed: (i) demineralisation of enamel in 3% citric acid and infiltration treatment with infiltration resin (Icon, DMG), remineralisation with Fluor Protector (Ivoclar Vivadent) and artificial saliva pH 8; and (ii) enamel demineralisation in saliva at pH 3 and remineralisation at salivary pH 8. The results showed that, firstly, for the remineralisation of demineralised enamel samples, Fluor Protector (Ivoclar Vivadent) was very effective for medium demineralised lesions followed by saliva remineralisation. In cases of deep demineralisation lesions where fluoride could not penetrate, low viscosity resin (Icon, DMG, Hamburg) effectively infiltrated to stop the demineralisation process. Secondly, remineralisation in salivary conditions needed supplementary study over a longer period, to analyse the habits, diet and nutrition of patients in detail. Finally, demineralisation/remineralisation processes were found to influence the macro elemental composition of enamel demineralisation, with natural saliva proving to be less aggressive in terms of decreasing Ca and Mg content.
本研究的目的是使用不同的牙科材料评估唾液再矿化与牙釉质化学再矿化/渗透的效果。使用共聚焦激光扫描显微镜(CLSM)研究牙釉质的变化,并计算病变深度和脱矿/再矿化/渗透百分比。此外,使用原子吸收光谱法(AAS)对牙齿的宏观元素组成进行分析。进行了两项研究:(i)在3%柠檬酸中使牙釉质脱矿,并用渗透树脂(Icon,DMG)进行渗透处理,用氟保护漆(Ivoclar Vivadent)和pH值为8的人工唾液进行再矿化;(ii)在pH值为3的唾液中使牙釉质脱矿,并在唾液pH值为8时进行再矿化。结果表明,首先,对于脱矿牙釉质样本的再矿化,氟保护漆(Ivoclar Vivadent)对中度脱矿病变非常有效,其次是唾液再矿化。在氟化物无法渗透的深度脱矿病变情况下,低粘度树脂(Icon,DMG,汉堡)能有效渗透以阻止脱矿过程。其次,唾液条件下的再矿化需要更长时间的补充研究,以详细分析患者的习惯、饮食和营养情况。最后,发现脱矿/再矿化过程会影响牙釉质脱矿的宏观元素组成,就降低钙和镁含量而言,天然唾液的侵蚀性较小。