Scholtens Petra A M J, Alles Martine S, Bindels Jacques G, van der Linde Esmeralda G M, Tolboom Jules J M, Knol Jan
Numico Research B.V., Bosrandweg 20, 6704 PH, Wageningen, The Netherlands.
J Pediatr Gastroenterol Nutr. 2006 May;42(5):553-9. doi: 10.1097/01.mpg.0000221887.28877.c7.
Breast-fed infants have relatively high proportions of faecal bifidobacteria. This bifidogenic microbiota is at least partly explained by the high levels of prebiotic oligosaccharides in human milk. The present study aimed at testing the effect of solid foods with added prebiotic galacto- and fructo-oligosaccharides (GOS/FOS) on the composition of the intestinal microbiota of fully formula-fed infants during the weaning period.
The study was a double-blind, randomised trial with an intervention period of 6 weeks. Infants aged 4 to 6 months who were about to start consuming solid foods were randomised to receive either weaning products with a mixture of GOS/FOS or control weaning products with maltodextrin. After an adjustment period, the presented daily dose of GOS/FOS was 4.5 g. Total numbers of bacteria and bifidobacteria in faecal samples were analysed with fluorescent in situ hybridization.
Thirty-five infants were included in the study, and 20 infants were included in a per-protocol analysis. In the GOS/FOS group, the faecal percentage of bifidobacteria significantly increased from 43% to 57% (P = 0.031) from week 0 to week 6, but did not significantly change in the control group (36% and 32%, respectively; P = 0.387). The change in the percentage of bifidobacteria between week 0 and week 6 in the GOS/FOS was significantly different from this change in the control group (P = 0.026).
We conclude that addition of GOS/FOS to solid foods induces an increase in the faecal proportion of bifidobacteria in the intestinal microbiota of fully formula-fed infants with an established, mixed-type microbiota in the weaning period.
母乳喂养的婴儿粪便中双歧杆菌比例相对较高。这种双歧杆菌丰富的微生物群至少部分是由母乳中高水平的益生元低聚糖所导致。本研究旨在测试添加益生元低聚半乳糖和低聚果糖（GOS/FOS）的固体食物对完全配方奶喂养婴儿在断奶期肠道微生物群组成的影响。
本研究为双盲随机试验，干预期为6周。即将开始食用固体食物的4至6个月大婴儿被随机分为两组，一组接受含有GOS/FOS混合物的断奶产品，另一组接受含有麦芽糊精的对照断奶产品。经过调整期后，每日提供的GOS/FOS剂量为4.5克。通过荧光原位杂交分析粪便样本中细菌和双歧杆菌的总数。
35名婴儿纳入研究，20名婴儿纳入符合方案分析。在GOS/FOS组中，从第0周到第6周，粪便中双歧杆菌的百分比从43%显著增加到57%（P = 0.031），而对照组没有显著变化（分别为36%和32%；P = 0.387）。GOS/FOS组第0周和第6周双歧杆菌百分比的变化与对照组的变化有显著差异（P = 0.026）。
我们得出结论，在固体食物中添加GOS/FOS可使断奶期已建立混合型微生物群的完全配方奶喂养婴儿肠道微生物群中粪便双歧杆菌比例增加。
