Athias P, Vandroux D, Tissier C, Rochette L
Laboratoire de physiopathologie et pharmacologie cardiovasculaires expérimentales (LPPCE), IFR no 100, institut de recherche cardiovasculaire, CHU le Bocage, 2, boulevard Maréchal-de-Lattre-de-Tassigny, BP 77908, 21079 Dijon, France.
Ann Cardiol Angeiol (Paris). 2006 Apr;55(2):90-9. doi: 10.1016/j.ancard.2006.02.004.
The cultures of neonatal rat cardiomyocytes represent a very useful tool for the observation and the understanding of the cellular aspects of the electrophysiological, contractile, morphological, metabolic and molecular properties of the myocardium. This model is characterized by a homogeneous population of cardiac muscular cells and by vast possibilities of control of the chemical and physical environment of the cells, allowing the in vitro mimicry of a wide range of cardiac pathological situations. The cardiomyocyte cultures are thus suited to very varied experimental protocols, allowing multiparametric analysis of the cardiocellular effects of different stress such as hypoxia-reoxygenation, of ischemia-reperfusion, of the free radical attack and of thermal shock. These investigations can be combined with the study of the effects and of the cytotoxicity of pharmacological agents, not limited to the putatively cardioactive drugs. The present review proposes an outline of the procedures for the isolation, the culture and the use of neonatal cardiomyocytes. To illustrate the potentialities of this preparation, we describe more specifically the protocols and the various consequences at the cellular scale of an in vitro model of myocardial ischemia reperfusion.
新生大鼠心肌细胞培养是观察和理解心肌电生理、收缩、形态、代谢及分子特性等细胞层面特征的非常有用的工具。该模型的特点是心肌细胞群体均匀，并且对细胞的化学和物理环境具有广泛的控制可能性，能够在体外模拟多种心脏病理情况。因此，心肌细胞培养适用于非常多样的实验方案，可对不同应激（如缺氧-复氧、缺血-再灌注、自由基攻击和热休克）的心肌细胞效应进行多参数分析。这些研究可以与药物制剂的作用及细胞毒性研究相结合，且不限于假定具有心脏活性的药物。本综述概述了新生心肌细胞的分离、培养和使用方法。为说明该制备方法的潜力，我们更具体地描述了心肌缺血再灌注体外模型的方案及其在细胞水平上的各种后果。
