Chlopcíková S, Psotová J, Miketová P
Institute of Medical Chemistry and Biochemistry, Medical Faculty of Palacký University, 775 15 Olomouc, Czech Republic.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2001 Dec;145(2):49-55.
The neonatal rat cardiomyocyte model enables heart researchers to study and understand the morphological, biochemical and electrophysiological characteristics of the heart. This model offers a broad spectrum of experiments, such as studies of contraction, ischaemia, hypoxia and the toxicity of various compounds. This review examines the methodology for the isolation and cultivation of primary neonatal rat cardiomyocyte cultures, considers possible problems and pitfalls, and presents an optimized protocol for cardiomyocyte preparation.
新生大鼠心肌细胞模型使心脏研究人员能够研究和了解心脏的形态、生化和电生理特征。该模型提供了广泛的实验，如收缩、缺血、缺氧及各种化合物毒性的研究。本文综述了原代新生大鼠心肌细胞培养的分离和培养方法，考虑了可能出现的问题和陷阱，并提出了优化的心肌细胞制备方案。
